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Literature summary extracted from

  • Isono, Y.; Sudo, T.; Hoshino, M.
    Properties of a new enzyme, nucleoside oxidase, from Pseudomonas maltophilia LB-86 (1989), Agric. Biol. Chem., 53, 1671-1677.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.28 HgCl2 30.8% inhibition at 1 mM Stenotrophomonas maltophilia
1.1.3.28 KCN 65.2% inhibition at 1 mM, complete inhibition at 10 mM Stenotrophomonas maltophilia
1.1.3.28 N-bromosuccinimide complete inhibition at 1 mM Stenotrophomonas maltophilia
1.1.3.28 NaN3 18.5% inhibition at 1 mM Stenotrophomonas maltophilia
1.1.3.28 Pb(CH3COO)2 18.7% inhibition at 1 mM Stenotrophomonas maltophilia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.28 0.044
-
 Inosine
-
 Stenotrophomonas maltophilia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.28 Fe2+ 2 g non-heme iron per mol of enzyme, contains also a heme component Stenotrophomonas maltophilia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.28 14000
-
 alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds Stenotrophomonas maltophilia
1.1.3.28 18000
-
 alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds Stenotrophomonas maltophilia
1.1.3.28 33000
-
 alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds Stenotrophomonas maltophilia
1.1.3.28 76000
-
 alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds Stenotrophomonas maltophilia
1.1.3.28 130000
-
 gel filtration Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.28 inosine + O2 Stenotrophomonas maltophilia oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose 9-riburonosylhypoxanthine + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.28 Stenotrophomonas maltophilia
-
-
-
1.1.3.28 Stenotrophomonas maltophilia LB-86
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.28 to homogeneity, chromatography techniques Stenotrophomonas maltophilia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.28 cell culture
-
 Stenotrophomonas maltophilia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.28 2'-deoxyadenosine + O2 81.4% of the activity compared to inosine Stenotrophomonas maltophilia 9-(2-deoxy-beta-D-erythro-pentofuranuronosyl)-9H-purin-6-amine + H2O
-
 ?
1.1.3.28 adenosine + O2 97.3% of the activity compared to inosine Stenotrophomonas maltophilia 9-riburonosyladenine + H2O
-
 ?
1.1.3.28 adenosine + O2 97.3% of the activity compared to inosine Stenotrophomonas maltophilia LB-86 9-riburonosyladenine + H2O
-
 ?
1.1.3.28 cytidine + O2 77.3% of the activity compared to inosine Stenotrophomonas maltophilia 1-riburonosylcytosine + H2O
-
 ?
1.1.3.28 cytidine + O2 77.3% of the activity compared to inosine Stenotrophomonas maltophilia LB-86 1-riburonosylcytosine + H2O
-
 ?
1.1.3.28 deoxycytidine + O2 77.3% of the activity compared to inosine Stenotrophomonas maltophilia 1-riburonosylcytosine + H2O
-
 ?
1.1.3.28 deoxyguanosine + O2 92.3% of the activity compared to inosine Stenotrophomonas maltophilia 9-riburonosyldeoxyguanine + H2O
-
 ?
1.1.3.28 deoxyinosine + O2 80.7% of the activity compared to inosine Stenotrophomonas maltophilia 9-riburonosyldeoxyhypoxanthine + H2O
-
 ?
1.1.3.28 guanosine + O2 121% of the activity compared to inosine Stenotrophomonas maltophilia 9-riburonosylguanine + H2O
-
 ?
1.1.3.28 guanosine + O2 121% of the activity compared to inosine Stenotrophomonas maltophilia LB-86 9-riburonosylguanine + H2O
-
 ?
1.1.3.28 inosine + O2 oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose Stenotrophomonas maltophilia 9-riburonosylhypoxanthine + H2O
-
 ?
1.1.3.28 thymidine + O2 55.8% of the activity compared to inosine Stenotrophomonas maltophilia 1-riburonosylthymine + H2O
-
 ?
1.1.3.28 uridine + O2
-
 Stenotrophomonas maltophilia 1-riburonosyluracil + H2O
-
 ?
1.1.3.28 uridine + O2
-
 Stenotrophomonas maltophilia LB-86 1-riburonosyluracil + H2O
-
 ?
1.1.3.28 xanthosine + O2 best substrate tested, 125% of the activity compared to inosine Stenotrophomonas maltophilia 9-riburonosylxanthine + H2O
-
 ?
1.1.3.28 xanthosine + O2 best substrate tested, 125% of the activity compared to inosine Stenotrophomonas maltophilia LB-86 9-riburonosylxanthine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.28 heterotetramer alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds Stenotrophomonas maltophilia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.28 60
-
 stable below, complete loss of activity at 75°C Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.28 5 6
-
 Stenotrophomonas maltophilia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.28 5 6 stable for 1 h at 37°C Stenotrophomonas maltophilia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.28 FAD 1 FAD covalently bound to the alpha subunit Stenotrophomonas maltophilia