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Literature summary extracted from

  • Tani, A.; Sakai, Y.; Ishige, T.; Kato, N.
    Thermostable NADP(+)-dependent medium-chain alcohol dehydrogenase from Acinetobacter sp. strain M-1: purification and characterization and gene expression in Escherichia coli (2000), Appl. Environ. Microbiol., 66, 5231-5235.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.2 hexadecane induces enzyme Acinetobacter sp.

Application

EC Number Application Comment Organism
1.1.1.2 synthesis develpoment of conversion processes for petrochemicals and oil-contaminated environments, cinnamyl aldehyde and cinnamyl alcohol used in flavor and perfume industry, anisaldehyde is used for perfume and toilet soaps, decylalcohol is used in the manufacture of plasticizers, a production system for this enzyme may be useful for industrial application as a biocatalyst in the future Acinetobacter sp.
1.1.1.2 synthesis reduction of industrially important compounds cinnamyl aldehyde and anisaldehyde, industrial bioconversion of useful alcohols and aldehydes Acinetobacter sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.2 alrA gene PCR amplified, cloned and highly expressed in Escherichia coli JM109 Acinetobacter sp.

General Stability

EC Number General Stability Organism
1.1.1.2 unstable Acinetobacter calcoaceticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.2 Ag+ 100% inhibition Acinetobacter sp.
1.1.1.2 Ca2+ 26% inhibition Acinetobacter sp.
1.1.1.2 Co2+ 91% inhibition Acinetobacter sp.
1.1.1.2 Hg2+ 100% inhibition Acinetobacter sp.
1.1.1.2 iodoacetamide
-
 Acinetobacter sp.
1.1.1.2 Mn2+ 29% inhibition Acinetobacter sp.
1.1.1.2 N-ethylmaleimide
-
 Acinetobacter sp.
1.1.1.2 Ni2+ 84% inhibition Acinetobacter sp.
1.1.1.2 p-mercuribenzoate
-
 Acinetobacter sp.
1.1.1.2 Zn2+ 100% inhibition Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 40000
-
 4 * 40000, SDS-PAGE Acinetobacter sp.
1.1.1.2 160000
-
 gel filtration Acinetobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Acinetobacter calcoaceticus
-
 strainNCIB8250, strain HO1-N
-
1.1.1.2 Acinetobacter sp.
-
-
-
1.1.1.2 Acinetobacter sp. M-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2
-
 Acinetobacter calcoaceticus
1.1.1.2 wild-type and recombinant protein Acinetobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 1.7
-
 recombinant enzyme, expressed in Escherichia coli JM109 Acinetobacter sp.
1.1.1.2 560
-
-
 Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 2,4-hexadienal + NADPH
-
 Acinetobacter sp. 2,4-hexadien-1-ol + NADP+
-
 ?
1.1.1.2 2-decanone + NADPH
-
 Acinetobacter sp. 2-decanol + NADP+
-
 ?
1.1.1.2 2-methylundecanal + NADPH
-
 Acinetobacter sp. 2-methylundecanol + NADP+
-
 ?
1.1.1.2 3-heptanone + NADPH
-
 Acinetobacter sp. 3-heptanol + NADP+
-
 ?
1.1.1.2 3-octanone + NADPH
-
 Acinetobacter sp. 3-octanol + NADP+
-
 ?
1.1.1.2 acetaldehyde + NADPH + H+
-
 Acinetobacter sp. ethanol + NADP+
-
 ?
1.1.1.2 acetaldehyde + NADPH + H+
-
 Acinetobacter sp. M-1 ethanol + NADP+
-
 ?
1.1.1.2 anisaldehyde + NADPH + H+
-
 Acinetobacter sp. anisyl alcohol + NADP+
-
 r
1.1.1.2 benzaldehyde + NADPH
-
 Acinetobacter sp. benzyl alcohol + NADP+
-
 r
1.1.1.2 benzaldehyde + NADPH
-
 Acinetobacter sp. M-1 benzyl alcohol + NADP+
-
 r
1.1.1.2 hexadecanol + NADP+
-
 Acinetobacter calcoaceticus hexadecanal + NADPH
-
 r
1.1.1.2 hexanal + NADPH + H+
-
 Acinetobacter sp. hexanol + NADP+
-
 r
1.1.1.2 hexanal + NADPH + H+
-
 Acinetobacter sp. M-1 hexanol + NADP+
-
 r
1.1.1.2 m-fluorobenzaldehyde + NADPH + H+
-
 Acinetobacter sp. m-fluorobenzyl alcohol + NADP+
-
 ?
1.1.1.2 m-methylbenzaldehyde + NADPH
-
 Acinetobacter sp. m-methylbenzyl alcohol + NADP+
-
 ?
1.1.1.2 n-butyraldehyde + NADPH
-
 Acinetobacter sp. n-butanol + NADP+
-
 r
1.1.1.2 n-decanal + NADPH + H+
-
 Acinetobacter sp. n-decanol + NADP+
-
 r
1.1.1.2 n-dodecanal + NADPH + H+
-
 Acinetobacter sp. n-dodecanol + NADP+
-
 r
1.1.1.2 n-heptanal + NADPH
-
 Acinetobacter sp. heptanol + NADP+
-
 r
1.1.1.2 n-octanal + NADPH + H+
-
 Acinetobacter sp. n-octanol + NADP+
-
 r
1.1.1.2 nonanal + NADPH
-
 Acinetobacter sp. ? + NADP+
-
 ?
1.1.1.2 o-fluorobenzaldehyde + NADPH + H+
-
 Acinetobacter sp. o-fluorobenzyl alcohol + NADP+
-
 ?
1.1.1.2 o-methylbenzaldehyde + NADPH
-
 Acinetobacter sp. o-methylbenzyl alcohol + NADP+
-
 ?
1.1.1.2 p-fluorobenzaldehyde + NADPH + H+
-
 Acinetobacter sp. p-fluorobenzyl alcohol + NADP+
-
 ?
1.1.1.2 p-methylbenzaldehyde + NADPH
-
 Acinetobacter sp. p-methylbenzyl alcohol + NADP+
-
 ?
1.1.1.2 pentanal + NADPH
-
 Acinetobacter sp. pentanol + NADP+
-
 r
1.1.1.2 pentanal + NADPH
-
 Acinetobacter sp. M-1 pentanol + NADP+
-
 r
1.1.1.2 phenylacetaldehyde + NADPH + H+
-
 Acinetobacter sp. phenylethanol + NADP+
-
 r
1.1.1.2 phenylacetaldehyde + NADPH + H+
-
 Acinetobacter sp. M-1 phenylethanol + NADP+
-
 r
1.1.1.2 propanal + NADPH + H+
-
 Acinetobacter sp. propanol + NADP+
-
 r
1.1.1.2 tetradecanal + NADPH
-
 Acinetobacter sp. tetradecanol
-
 r
1.1.1.2 trans-2-decenal + NADPH
-
 Acinetobacter sp. trans-2-decen-1-ol + NADP+
-
 ?
1.1.1.2 trans-cinnamaldehyde + NADPH
-
 Acinetobacter sp. cinnamyl alcohol + NADP+
-
 ?
1.1.1.2 tridecanal + NADPH
-
 Acinetobacter sp. tridecanol + NADP+
-
 r
1.1.1.2 undecanal + NADPH
-
 Acinetobacter sp. undecanol + NADP+
-
 ?
1.1.1.2 vitamin A aldehyde + NADPH
-
 Acinetobacter sp. ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.2 tetramer 4 * 40000, SDS-PAGE Acinetobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.2 70
-
 retains 90% activity after 40 min Acinetobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 7
-
 n-heptanal reduction Acinetobacter sp.
1.1.1.2 9.5
-
 n-heptanol oxidation Acinetobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.2 8 9
-
 Acinetobacter sp.