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Literature summary extracted from

  • MacDonald, I.A.; Williams, C.N.; Mahony, D.E.
    7alpha-hydroxysteroid dehydrogenase from Escherichia coli B: preliminary studies (1973), Biochim. Biophys. Acta, 309, 243-253.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.159 inactivation by freezing Escherichia coli
1.1.1.159 stabilization by glutathione, EDTA Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.159 chenodeoxycholate substrate inhibition at concentration above 1 mM Escherichia coli
1.1.1.159 glycochenodeoxycholate substrate inhibition at concentration above 1 mM Escherichia coli
1.1.1.159 taurochenodeoxycholate substrate inhibition at concentration above 1 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.159 0.06
-
chenodeoxycholic acid
-
Escherichia coli
1.1.1.159 0.085
-
glycochenodeoxycholic acid
-
Escherichia coli
1.1.1.159 0.24
-
taurochenodeoxycholic acid
-
Escherichia coli
1.1.1.159 0.8
-
cholic acid
-
Escherichia coli
1.1.1.159 1
-
taurocholic acid
-
Escherichia coli
1.1.1.159 1
-
glycocholic acid
-
Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.159 bile acids + NAD(P)+ Escherichia coli
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 bile acids + NAD(P)+
-
Escherichia coli ?
-
?
1.1.1.159 chenodeoxycholic acid + NAD(P)+ i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid Escherichia coli 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
?
1.1.1.159 cholic acid + NAD(P)+
-
Escherichia coli 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
?
1.1.1.159 glycochenodeoxycholic acid + NAD(P)+
-
Escherichia coli N-[3alpha-hydroxy-7,24-dioxocholan-24-yl]glycine + NAD(P)H
-
?
1.1.1.159 glycocholic acid + NAD+ i.e. N-[3alpha,7alpha,12alpha-trihydroxy-24-oxocholan-24-yl]glycine Escherichia coli N-[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]glycine + NADH
-
?
1.1.1.159 taurochenodeoxycholic acid + NAD+
-
Escherichia coli 2[[3alpha-hydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
?
1.1.1.159 taurocholic acid + NAD+ i.e. 2[[3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholan-24-yl]amino]ethane sulfonic acid Escherichia coli 2[[3alpha,12alpha-dihydroxy-7,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADH
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.159 9 10 oxidation of cholic acid Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.159 NAD+
-
Escherichia coli