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Literature summary extracted from

  • Gasson, M.J.; Kitamura, Y.; McLauchlan, W.R.; Narbad, A.; Parr, A.J.; Parsons, E.L.; Payne, J.; Rhodes, M.J.; Walton, N.J.
    Metabolism of ferulic acid to vanillin. A bacterial gene of the enoyl-SCoA hydratase/isomerase superfamily encodes an enzyme for the hydration and cleavage of a hydroxycinnamic acid SCoA thioester (1998), J. Biol. Chem., 273, 4163-4170.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.61 synthesis biotechnological production of vanillin Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.61 gene encoding an enoyl-SCoA hydratase/lyase isolated, cloned and heterologously expressed in Escherichia coli JM109 Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.61 31000
-
 gel filtration Pseudomonas fluorescens
4.1.2.61 31000
-
 alpha, alpha, 2 * 31000, SDS-PAGE, deduced from gene sequence Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA Pseudomonas fluorescens
-
 vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA Delftia acidovorans
-
 vanillin + acetyl-CoA
-
 ?
4.1.2.61 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA Pseudomonas fluorescens
-
 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O Pseudomonas fluorescens
-
 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.61 Delftia acidovorans
-
-
-
4.1.2.61 Pseudomonas fluorescens
-
-
-
4.1.2.61 Pseudomonas fluorescens
-
 biovar V, strain AN103
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Pseudomonas fluorescens 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Pseudomonas fluorescens vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Delftia acidovorans vanillin + acetyl-CoA
-
 ?
4.1.2.61 4-hydroxy-3-methoxy-phenyl-beta-hydroxypropionyl-CoA + H2O
-
 Pseudomonas fluorescens vanillin + acetylCoA + feruloylCoA
-
 ?
4.1.2.61 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 Pseudomonas fluorescens 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 feruloyl-CoA + H2O
-
 Pseudomonas fluorescens vanillin + acetyl-CoA
-
 ?
4.1.2.61 feruloyl-CoA + H2O
-
 Delftia acidovorans vanillin + acetyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O
-
 Pseudomonas fluorescens 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.61 dimer alpha, alpha, 2 * 31000, SDS-PAGE, deduced from gene sequence Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
4.1.2.61 4-hydroxycinnamoyl-CoA hydratase/lyase
-
 Pseudomonas fluorescens