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Literature summary extracted from

  • Mitra, A.; Kitamura, Y.; Gasson, M.J.; Narbad, A.; Parr, A.J.; Pyne, J.; Rhodes, M.J.C.; Sewter, C.; Walton, N.J.
    4-Hydroxycinnamoyl-CoA hydratase/lyase (HCHL)-an enzyme of phenylpropanoid chain cleavage from Pseudomonas (1999), Arch. Biochem. Biophys., 365, 10-16.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.61 expressed in Escherichia coli Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.61 1.6
-
 caffeoyl-CoA
-
 Pseudomonas fluorescens
4.1.2.61 2.4
-
 feruloyl-CoA
-
 Pseudomonas fluorescens
4.1.2.61 5.2
-
 4-coumaroyl-CoA
-
 Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.61 31000
-
 alpha, alpha, 2 * 31000, SDS-PAGE, deduced from gene sequence Pseudomonas fluorescens
4.1.2.61 63000
-
 gel filtration Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.61 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA Pseudomonas fluorescens
-
 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA Pseudomonas fluorescens AN103
-
 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O Pseudomonas fluorescens
-
 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O Pseudomonas fluorescens AN103
-
 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.61 Pseudomonas fluorescens
-
-
-
4.1.2.61 Pseudomonas fluorescens AN103
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.61 Mono-Q, Mono-P, Phenyl-Superose Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.61 0.003
-
-
 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
4.1.2.61 -70°C, several months Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.61 3,4-dihydroxycinnamoyl-CoA + H2O
-
 Pseudomonas fluorescens 3,4-dihydroxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 3,4-dihydroxycinnamoyl-CoA + H2O
-
 Pseudomonas fluorescens AN103 3,4-dihydroxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 Pseudomonas fluorescens 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 Pseudomonas fluorescens AN103 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 4-hydroxycinnamoyl-CoA + H2O
-
 Pseudomonas fluorescens 4-hydroxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 4-hydroxycinnamoyl-CoA + H2O
-
 Pseudomonas fluorescens AN103 4-hydroxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O
-
 Pseudomonas fluorescens 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?
4.1.2.61 trans-feruloyl-CoA + H2O
-
 Pseudomonas fluorescens AN103 4-hydroxy-3-methoxyphenyl-beta-hydroxypropionyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.61 dimer alpha, alpha, 2 * 31000, SDS-PAGE, deduced from gene sequence Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
4.1.2.61 4-hydroxycinnamoyl-CoA hydratase/lyase enzyme has hydratase and lyase activity Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.61 2.3
-
 4-coumaroyl-CoA at 30°C Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.61 8.5 9.5 in Tris-HCl or borate buffer, activity declined to ca. 50% of its maximum at pH 6.5 Pseudomonas fluorescens