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Literature summary extracted from

  • Beller, H.R.; Spormann, A.M.
    Substrate range of benzylsuccinate synthase from Azoarcus sp. strain T (1999), FEMS Microbiol. Lett., 178, 147-153.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.11 6500
-
 alpha, alpha, beta, gamma, 1 * 97000 + 1 * 97000 + 1 * 14500 + 1 * 6500, SDS-PAGE Azoarcus sp.
4.1.99.11 14500
-
 alpha, alpha, beta, gamma, 1 * 97000 + 1 * 97000 + 1 * 14500 + 1 * 6500, SDS-PAGE Azoarcus sp.
4.1.99.11 97000
-
 alpha, alpha, beta, gamma, 1 * 97000 + 1 * 97000 + 1 * 14500 + 1 * 6500, SDS-PAGE Azoarcus sp.
4.1.99.11 260000
-
 gel filtration Azoarcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.11 toluene + fumarate Azoarcus sp.
-
 benzylsuccinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.11 Azoarcus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.11 hydroxyapatite, gel filtration, 95% loss of specific activity after gel filtration Azoarcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.11 0.003
-
-
 Azoarcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.11 2-fluorotoluene + fumarate
-
 Azoarcus sp. (2-fluorobenzyl)succinate
-
 ?
4.1.99.11 3-fluorotoluene + fumarate
-
 Azoarcus sp. (3-fluorobenzyl)succinate
-
 ?
4.1.99.11 4-fluorotoluene + fumarate
-
 Azoarcus sp. (4-fluorobenzyl)succinate
-
 ?
4.1.99.11 benzaldehyde + fumarate
-
 Azoarcus sp. benzoylsuccinate
-
 ?
4.1.99.11 m-xylene-d6 + fumarate
-
 Azoarcus sp. (3-methylbenzyl)succinate-d6
-
 ?
4.1.99.11 o-xylene-d10 + fumarate
-
 Azoarcus sp. (2-methylbenzyl)succinate-d10
-
 ?
4.1.99.11 p-xylene-d10 + fumarate
-
 Azoarcus sp. (4-methylbenzyl)succinate-d10
-
 ?
4.1.99.11 toluene + fumarate
-
 Azoarcus sp. benzylsuccinate
-
 ?
4.1.99.11 toluene + maleate
-
 Azoarcus sp. benzylsuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.11 tetramer alpha, alpha', beta, gamma, 1 * 97000 + 1 * 97000 + 1 * 14500 + 1 * 6500, SDS-PAGE Azoarcus sp.