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Literature summary extracted from

  • Bhalla, T.C.; Miura, A.; Wakamoto, A.; Ohba, Y.; Furuhashi, K.
    Asymmetric hydrolysis of alpha-aminonitriles to optically active amino acids by a nitrilase of Rhodococcus rhodochrous PA-34 (1992), Appl. Microbiol. Biotechnol., 37, 184-190.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.5.1 hydroxylamine 20% activation at 1 mM Rhodococcus rhodochrous

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 4-chloromercuribenzoate nearly complete inhibition at 1 mM Rhodococcus rhodochrous
3.5.5.1 5,5'-dithiobis(2-nitrobenzoic acid) complete inhibition at 1 mM Rhodococcus rhodochrous
3.5.5.1 Ag+ complete inhibition at 5 mM Rhodococcus rhodochrous
3.5.5.1 Co2+ complete inhibition at 1 mM Rhodococcus rhodochrous
3.5.5.1 Hg2+ 43% inhibition at 1 mM Rhodococcus rhodochrous
3.5.5.1 iodoacetic acid slight inhibition at 1 mM Rhodococcus rhodochrous
3.5.5.1 o-phenanthroline complete inhibition at 5 mM Rhodococcus rhodochrous
3.5.5.1 Zn2+ complete inhibition at 1 mM Rhodococcus rhodochrous

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 45000
-
 gel filtration Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 acrylonitrile + H2O Rhodococcus rhodochrous
-
 acrylic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O Rhodococcus rhodochrous best substrate benzoic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Rhodococcus rhodochrous
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 to homogeneity, chromatography techniques Rhodococcus rhodochrous

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.1 cell culture best inducer of enzyme formation: isobutyronitrile Rhodococcus rhodochrous
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.1 3.52
-
-
 Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 2-aminobutyronitrile + H2O
-
 Rhodococcus rhodochrous 2-aminobutyric acid + NH3
-
 ?
3.5.5.1 2-aminopropionitrile + H2O low activity Rhodococcus rhodochrous 2-aminopropionic acid + NH3
-
 ?
3.5.5.1 2-methylbutyronitrile + H2O
-
 Rhodococcus rhodochrous 2-methylbutyric acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O
-
 Rhodococcus rhodochrous acrylic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O best substrate Rhodococcus rhodochrous benzoic acid + NH3
-
 ?
3.5.5.1 crotononitrile + H2O
-
 Rhodococcus rhodochrous NH3 + (z)-but-2-en-1-ol
-
 ?
3.5.5.1 DL-alpha-amino-4-methylthio butyronitrile + H2O
-
 Rhodococcus rhodochrous L-methionine + NH3
-
 ?
3.5.5.1 DL-alpha-amino-n-capronitrile + H2O
-
 Rhodococcus rhodochrous L-norleucine + NH3
-
 ?
3.5.5.1 DL-alpha-amino-n-valeronitrile + H2O
-
 Rhodococcus rhodochrous L-norvaline + NH3
-
 ?
3.5.5.1 DL-alpha-aminoisocapronitrile + H2O
-
 Rhodococcus rhodochrous L-leucine + NH3
-
 ?
3.5.5.1 DL-alpha-aminoisovaleronitrile + H2O
-
 Rhodococcus rhodochrous L-valine + NH3
-
 ?
3.5.5.1 DL-alpha-aminopropionitrile + H2O
-
 Rhodococcus rhodochrous D-alanine + NH3
-
 ?
3.5.5.1 lactonitrile + H2O
-
 Rhodococcus rhodochrous lactate + NH3
-
 ?
3.5.5.1 mandelonitrile + 2 H2O
-
 Rhodococcus rhodochrous mandelic acid + NH3
-
 ?
3.5.5.1 methacrylonitrile + H2O low activity Rhodococcus rhodochrous methylacrylic acid + NH3
-
 ?
3.5.5.1 n-butyronitrile + H2O
-
 Rhodococcus rhodochrous n-butyric acid + NH3
-
 ?
3.5.5.1 phenylacetonitrile + 2 H2O low activity Rhodococcus rhodochrous phenylacetate + NH3
-
 ?
3.5.5.1 phenylglycinonitrile + H2O low actiity Rhodococcus rhodochrous phenylglycine + NH3
-
 ?
3.5.5.1 propionitrile + H2O
-
 Rhodococcus rhodochrous propionic acid + NH3
-
 ?
3.5.5.1 succinonitrile + H2O low activity Rhodococcus rhodochrous succinate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 monomer 1 * 45000, SDS-PAGE Rhodococcus rhodochrous

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 35
-
-
 Rhodococcus rhodochrous

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.1 35
-
 decreasing activity above Rhodococcus rhodochrous

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 7.5
-
-
 Rhodococcus rhodochrous