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Literature summary extracted from

  • Nagawasa, T.; Wieser, M.; Nakamura, T.; Iwahara, H.; Yoshida, T.; Gekko, K.
    Nitrilase of Rhodococcus rhodochrous J1. Conversion into active form by subunit association (2000), Eur. J. Biochem., 267, 138-144.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 Hg2+ complete inhibition at 0.25 mM, also inhibition of enzyme association to decamers Rhodococcus rhodochrous

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 40000
-
 2 * 40000, SDS-PAGE, no hydrolysis of acrylonitrile Rhodococcus rhodochrous
3.5.5.1 40000
-
 10 * 40000, SDS-PAGE, hydrolysis of both benzonitrile and acrylonitrile after recovery conditions for acrylonitrile hydrolysis Rhodococcus rhodochrous
3.5.5.1 80000
-
 gel filtration, only active on benzonitrile Rhodococcus rhodochrous
3.5.5.1 412000
-
 sedimentation equilibrium analysis, after recovery conditions for hydrolysis of acrylonitrile Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 3-cyanopyridine + 2 H2O Rhodococcus rhodochrous lower activity for the purified enzyme compared to the activity in resting cells nicotinic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O Rhodococcus rhodochrous only hydrolysed after recovered activity by preincubation with benzonitrile or dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate acrylic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O Rhodococcus rhodochrous best substrate benzoic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Rhodococcus rhodochrous
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 to homogeneity, 2step chromatography Rhodococcus rhodochrous

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.5.1 cell culture induction of enzyme synthesis by epsilon-caprolactam- or isovaleronitrile-containing media Rhodococcus rhodochrous
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 3-cyanopyridine + 2 H2O lower activity for the purified enzyme compared to the activity in resting cells Rhodococcus rhodochrous nicotinic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O only hydrolysed after recovered activity by preincubation with benzonitrile or dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate Rhodococcus rhodochrous acrylic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O best substrate Rhodococcus rhodochrous benzoic acid + NH3
-
 ?
3.5.5.1 crotononitrile + H2O low activity Rhodococcus rhodochrous NH3 + (z)-but-2-en-1-ol
-
 ?
3.5.5.1 methacrylonitrile + H2O very low activity in resting cells and for the purified enzyme only after recovered activity by dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate Rhodococcus rhodochrous methylacrylic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 decamer 10 * 40000, SDS-PAGE, hydrolysis of both benzonitrile and acrylonitrile after recovery conditions for acrylonitrile hydrolysis Rhodococcus rhodochrous
3.5.5.1 dimer 2 * 40000, SDS-PAGE, no hydrolysis of acrylonitrile Rhodococcus rhodochrous