EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.4.5 | cdd gene cloned and amplified with pTK148, cloning vector pBR322, enzyme overexpression in Escherichia coli C600 | Escherichia coli |
3.5.4.5 | cdd genes cloned and characterized | Salmonella enterica subsp. enterica serovar Typhimurium |
3.5.4.5 | cdd genes cloned and characterized | Bacillus subtilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.5 | 3,4,5,6-Tetrahydrouridine | - |
Escherichia coli | |
3.5.4.5 | 5,6-Dihydrouridine | competitive inhibition | Escherichia coli | |
3.5.4.5 | 5-bromodeoxyuridine | - |
Escherichia coli | |
3.5.4.5 | adenosine | - |
Escherichia coli | |
3.5.4.5 | Cu2+ | - |
Escherichia coli | |
3.5.4.5 | deoxyadenosine | competitive inhibition | Escherichia coli | |
3.5.4.5 | deoxyguanosine | - |
Escherichia coli | |
3.5.4.5 | deoxyuridine | competitive inhibition | Escherichia coli | |
3.5.4.5 | Fe2+ | - |
Escherichia coli | |
3.5.4.5 | Fe3+ | - |
Escherichia coli | |
3.5.4.5 | guanosine | - |
Escherichia coli | |
3.5.4.5 | Hg+ | - |
Escherichia coli | |
3.5.4.5 | p-chloromercuribenzoate | - |
Saccharomyces cerevisiae | |
3.5.4.5 | thioglycolic acid | 0.7 mM 88% relative activity, complete inhibition at 7 mM | Escherichia coli | |
3.5.4.5 | thymidine | competitive inhibition | Escherichia coli | |
3.5.4.5 | uridine | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.5 | 0.0167 | - |
cytidine | nonhomogenous enzyme | Escherichia coli | |
3.5.4.5 | 0.089 | - |
cytosine deoxyriboside | - |
Escherichia coli | |
3.5.4.5 | 0.21 | - |
deoxycytidine | amplified gene | Escherichia coli | |
3.5.4.5 | 0.58 | - |
deoxycytidine | - |
Escherichia coli | |
3.5.4.5 | 2.2 | - |
cytidine | homogenous preparation | Escherichia coli | |
3.5.4.5 | 6.6 | - |
cytidine | amplified gene, pH 7.0, 37°C | Escherichia coli | |
3.5.4.5 | 8.53 | - |
deoxycytidine | - |
Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.4.5 | cytoplasm | inner-surface of cytoplasmic membrane | Escherichia coli | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.4.5 | 14000 | - |
4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments | Bacillus subtilis |
3.5.4.5 | 33000 | - |
2 * 33000, SDS-PAGE | Escherichia coli |
3.5.4.5 | 34000 | - |
2 * 34000, SDS-PAGE | Escherichia coli |
3.5.4.5 | 54000 | - |
- |
Escherichia coli |
3.5.4.5 | 56000 | - |
calculated from Stokes radius | Bacillus subtilis |
3.5.4.5 | 56000 | - |
calculated from Stokes radius | Escherichia coli |
3.5.4.5 | 57000 | - |
gel filtration, homogenous preparation | Escherichia coli |
3.5.4.5 | 62000 | - |
gel filtration, native enzyme | Escherichia coli |
3.5.4.5 | 73000 | - |
gel filtration, nonhomogenous preparation | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.5 | cytidine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
uridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | Bacillus subtilis | - |
uridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | Escherichia coli | - |
uridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | Saccharomyces cerevisiae | - |
uridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | Geobacillus stearothermophilus | - |
uridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | Escherichia coli JF611 | - |
uridine + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.5 | Bacillus subtilis | - |
- |
- |
3.5.4.5 | Escherichia coli | - |
- |
- |
3.5.4.5 | Escherichia coli JF611 | - |
- |
- |
3.5.4.5 | Geobacillus stearothermophilus | - |
- |
- |
3.5.4.5 | Saccharomyces cerevisiae | - |
bakers' yeast | - |
3.5.4.5 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.4.5 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.4.5 | 4 | - |
amplified gene, 5'-aza-2'-deoxycytidine as substrate | Escherichia coli |
3.5.4.5 | 4.9 | - |
amplified gene, 2'-thiocytidine as substrate | Escherichia coli |
3.5.4.5 | 23.9 | - |
amplified gene, 5'-methylcytidine as substrate | Escherichia coli |
3.5.4.5 | 31.8 | - |
amplified gene, ara-cytidine as substrate | Escherichia coli |
3.5.4.5 | 43 | - |
amplified gene, cytidine as substrate | Escherichia coli |
3.5.4.5 | 47.9 | - |
amplified gene, 5'-methyl-2'-deoxycytidine as substrate | Escherichia coli |
3.5.4.5 | 68.4 | - |
amplified gene, 2'-fluoro-2'-deoxycytidine as substrate | Escherichia coli |
3.5.4.5 | 97.6 | - |
amplified gene, deoxycytidine as substrate | Escherichia coli |
3.5.4.5 | 3558 | - |
amplified gene | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.5 | 1-beta-D-arabinofuranosylcytosine + H2O | - |
Escherichia coli | ? | - |
? | |
3.5.4.5 | 2'-deoxycytidine + H2O | - |
Escherichia coli | 2'-deoxyuridine + NH3 | - |
? | |
3.5.4.5 | 2'-deoxycytidine + H2O | - |
Saccharomyces cerevisiae | 2'-deoxyuridine + NH3 | - |
? | |
3.5.4.5 | 2'-deoxycytidine + H2O | - |
Escherichia coli JF611 | 2'-deoxyuridine + NH3 | - |
? | |
3.5.4.5 | 2'-fluoro-2'-deoxycytidine + H2O | - |
Escherichia coli | 2'-fluoro-2'-deoxyuridine + NH3 | - |
? | |
3.5.4.5 | 2'-fluoro-2'-deoxycytidine + H2O | - |
Escherichia coli JF611 | 2'-fluoro-2'-deoxyuridine + NH3 | - |
? | |
3.5.4.5 | 2'-thio-cytidine + H2O | - |
Escherichia coli | 2'-thiouridine + NH3 | - |
? | |
3.5.4.5 | 5'-methyl-2'-deoxycytidine + H2O | - |
Escherichia coli | 5'-methyl-2-deoxyuridine + NH3 | - |
? | |
3.5.4.5 | 5'-methyl-2'-deoxycytidine + H2O | - |
Escherichia coli JF611 | 5'-methyl-2-deoxyuridine + NH3 | - |
? | |
3.5.4.5 | 5-aza-2'-deoxycytidine + H2O | - |
Escherichia coli | 5-aza-2'-deoxyuridine + NH3 | - |
? | |
3.5.4.5 | 5-methylcytidine + H2O | - |
Escherichia coli | 5-methyluridine + NH3 | - |
? | |
3.5.4.5 | 5-methylcytidine + H2O | - |
Escherichia coli JF611 | 5-methyluridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | uridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | - |
Bacillus subtilis | uridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | - |
Escherichia coli | uridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | - |
Saccharomyces cerevisiae | uridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | - |
Geobacillus stearothermophilus | uridine + NH3 | - |
? | |
3.5.4.5 | cytidine + H2O | - |
Escherichia coli JF611 | uridine + NH3 | - |
? | |
3.5.4.5 | cytosine deoxyriboside + H2O | - |
Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.4.5 | dimer | 2 * 34000, SDS-PAGE | Escherichia coli |
3.5.4.5 | dimer | 2 * 33000, SDS-PAGE | Escherichia coli |
3.5.4.5 | tetramer | 4 * 14000, deduced from cdd structural gene nucleotide sequence and mini-cell experiments | Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.4.5 | 30 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
3.5.4.5 | 30 | - |
- |
Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.4.5 | 60 | - |
relatively labile to thermal treatment, complete inactivation by incubation for 30 min | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.4.5 | 7 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
3.5.4.5 | 7 | - |
- |
Escherichia coli |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.4.5 | 6 | 9 | reasonably stable in this range | Escherichia coli |