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Literature summary extracted from
- Purification and characterization of a new hydrolase for conjugated bile acids, chenodeoxycholyltaurine hydrolase, from Bacteroides vulgatus (1989), J. Biochem., 106, 1049-1053.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.74 | dithiothreitol | 0.1-1 mM: slight activation, 10 mM: high activation | Phocaeicola vulgatus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.74 | dithiodipyridine | 0.1 mM: 15.9% inhibition, 1 mM: 50.2% inhibition, 10 mM: complete inhibition | Phocaeicola vulgatus | |
| 3.5.1.74 | N-ethylmaleimide | 0.1 mM: 3.5% inhibition, 1 mM: 27.4% inhibition, 10 mM: 90.8% inhibition | Phocaeicola vulgatus | |
| 3.5.1.74 | p-hydroxymercuribenzoate | 0.00001 mM: 4% inhibition, 0.0001 mM: 60% inhibition, 0.001 mM: complete inhibition | Phocaeicola vulgatus | |
| 3.5.1.74 | sulfhydryl reagents | - |
Phocaeicola vulgatus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.74 | additional information | - |
additional information | kinetic properties | Phocaeicola vulgatus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.74 | 36000 | - |
4 * 36000, SDS-PAGE | Phocaeicola vulgatus |
| 3.5.1.74 | 140000 | - |
gel filtration | Phocaeicola vulgatus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.74 | Phocaeicola vulgatus | - |
isolated from human feces | - |
| 3.5.1.74 | Phocaeicola vulgatus VI-31 | - |
isolated from human feces | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.74 | strain VI-31 | Phocaeicola vulgatus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.74 | 0.11 | - |
substrate: tauro-7beta-cholic acid | Phocaeicola vulgatus |
| 3.5.1.74 | 0.69 | - |
substrate: taurocholic acid | Phocaeicola vulgatus |
| 3.5.1.74 | 5.86 | - |
substrate: taurohyocholic acid | Phocaeicola vulgatus |
| 3.5.1.74 | 7.56 | - |
substrate: taurodeoxycholic acid | Phocaeicola vulgatus |
| 3.5.1.74 | 8.74 | - |
substrate: taurohyodeoxycholic acid | Phocaeicola vulgatus |
| 3.5.1.74 | 13.68 | - |
substrate: tauroursodeoxycholic acid | Phocaeicola vulgatus |
| 3.5.1.74 | 15.15 | - |
substrate: tauro-beta-muricholic acid | Phocaeicola vulgatus |
| 3.5.1.74 | 24.45 | - |
substrate: taurochenodeoxycholic acid | Phocaeicola vulgatus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.1.74 | -20°C, stable for 1 month without any loss of activity | Phocaeicola vulgatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.74 | additional information | substrate specificity | Phocaeicola vulgatus | ? | - |
? | |
| 3.5.1.74 | additional information | hydrolyzes taurine-conjugated bile acids, glycine-conjugated bile acids are no substrates | Phocaeicola vulgatus | ? | - |
? | |
| 3.5.1.74 | additional information | no activity with glycocholic acid, glyco-7beta-cholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, glyco-beta-muricholic acid, glycoursodeoxycholic acid, glycohyocholic acid, glycohyodeoxycholic acid | Phocaeicola vulgatus | ? | - |
? | |
| 3.5.1.74 | additional information | specificity for not only the amino acid moiety but also the steroidal moiety | Phocaeicola vulgatus | ? | - |
? | |
| 3.5.1.74 | additional information | substrate specificity | Phocaeicola vulgatus VI-31 | ? | - |
? | |
| 3.5.1.74 | additional information | hydrolyzes taurine-conjugated bile acids, glycine-conjugated bile acids are no substrates | Phocaeicola vulgatus VI-31 | ? | - |
? | |
| 3.5.1.74 | additional information | no activity with glycocholic acid, glyco-7beta-cholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, glyco-beta-muricholic acid, glycoursodeoxycholic acid, glycohyocholic acid, glycohyodeoxycholic acid | Phocaeicola vulgatus VI-31 | ? | - |
? | |
| 3.5.1.74 | additional information | specificity for not only the amino acid moiety but also the steroidal moiety | Phocaeicola vulgatus VI-31 | ? | - |
? | |
| 3.5.1.74 | tauro (3alpha,5beta,7beta,12alpha)-3,7,12-trihydroxycholan-24-oic amide + H2O | 0.5% of activity compared to taurochenodeoxycholic acid | Phocaeicola vulgatus | taurine + (3alpha,5beta,7beta,12alpha)-3,7,12-trihydroxycholan-24-oic acid | - |
? | |
| 3.5.1.74 | tauro-beta-muricholic acid + H2O | 62% of activity compared to taurochenodeoxycholic acid | Phocaeicola vulgatus | taurine + beta-muricholic acid | - |
? | |
| 3.5.1.74 | taurochenodeoxycholic acid + H2O | best substrate | Phocaeicola vulgatus | chenodeoxycholate + taurine | - |
? | |
| 3.5.1.74 | taurochenodeoxycholic acid + H2O | best substrate | Phocaeicola vulgatus VI-31 | chenodeoxycholate + taurine | - |
? | |
| 3.5.1.74 | taurocholic acid + H2O | 2.8% of activity compared to taurochenodeoxycholic acid | Phocaeicola vulgatus | taurine + cholic acid | - |
? | |
| 3.5.1.74 | taurodeoxycholic acid + H2O | 31% of activity compared to taurochenodeoxycholic acid | Phocaeicola vulgatus | taurine + deoxycholic acid | - |
? | |
| 3.5.1.74 | taurohyocholic acid + H2O | 24% of activity compared to taurochenodeoxycholic acid | Phocaeicola vulgatus | taurine + hyocholic acid | - |
? | |
| 3.5.1.74 | taurohyodeoxycholic acid + H2O | 36% of activity compared to taurochenodeoxycholic acid | Phocaeicola vulgatus | taurine + hyodeoxycholic acid | - |
? | |
| 3.5.1.74 | tauroursodeoxycholic acid + H2O | 56% of activity compared to taurochenodeoxycholic acid | Phocaeicola vulgatus | taurine + ursodeoxycholic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.74 | tetramer | 4 * 36000, SDS-PAGE | Phocaeicola vulgatus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.74 | CTH | - |
Phocaeicola vulgatus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.74 | 37 | - |
assay at | Phocaeicola vulgatus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.74 | 20 | - |
48 h stable at | Phocaeicola vulgatus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.74 | 5.6 | 6.4 | broad pH-optimum | Phocaeicola vulgatus |
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