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Literature summary extracted from

  • Wyborn N.R.; Scherr, D.J.; Jones, C.W.
    Purification, properties and heterologous expression of formamidase from Methylophilus methylotrophus (1994), Microbiology, 140, 191-195.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.49 expression in Escherichia coli Methylophilus methylotrophus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.49 2.1
-
 Formamide
-
 Methylophilus methylotrophus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.49 cytosol
-
 Methylophilus methylotrophus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.49 51000
-
 alpha2, 2 * 51000, SDS-PAGE Methylophilus methylotrophus
3.5.1.49 123000
-
 gel filtration Methylophilus methylotrophus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.49 Methylophilus methylotrophus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.49 to homogeneity Methylophilus methylotrophus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.49 cell culture induction of enzyme synthesis using formamide- or acetamide-containing media Methylophilus methylotrophus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.49 37.4
-
-
 Methylophilus methylotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.49 acetamide + H2O very low activity Methylophilus methylotrophus acetic acid + NH3
-
 ?
3.5.1.49 acrylamide + H2O very low activity Methylophilus methylotrophus acrylic acid + NH3
-
 ?
3.5.1.49 butyramide + H2O very low activity Methylophilus methylotrophus butyric acid + NH3
-
 ?
3.5.1.49 formamide + H2O
-
 Methylophilus methylotrophus formate + NH3 source of nitrogen for growth and contribution to energy economy of the cell ?
3.5.1.49 propionamide + H2O very low activity Methylophilus methylotrophus propionic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.49 dimer alpha2, 2 * 51000, SDS-PAGE Methylophilus methylotrophus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.49 60.7
-
 Formamide
-
 Methylophilus methylotrophus