Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Meijer, J.; Depierre, J.W.
    Properties of cytosolic epoxide hydrolase purified from the liver of untreated and clofibrate-treated mice. Characterization of optimal assay conditions, substrate specificity and effects of modulators on the catalytic activity (1985), Eur. J. Biochem., 150, 7-16.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.3.2.10 Aminotriazole slight activation Mus musculus
3.3.2.10 benzil
-
 Mus musculus
3.3.2.10 Clofibrate slight inhibition of enzyme from untreated mice, slight activation of the enzyme from clofibrate-treated mice Mus musculus
3.3.2.10 ellipticine
-
 Mus musculus
3.3.2.10 Harmane
-
 Mus musculus
3.3.2.10 iodoacetamide
-
 Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.10 4-Phenylchalcone oxide
-
 Mus musculus
3.3.2.10 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Mus musculus
3.3.2.10 alpha-naphthoflavone
-
 Mus musculus
3.3.2.10 beta-naphthoflavone
-
 Mus musculus
3.3.2.10 Chalcone oxide
-
 Mus musculus
3.3.2.10 Clofibrate slight inhibition of enzyme from untreated mice, slight activation of the enzyme from clofibrate-treated mice Mus musculus
3.3.2.10 Dimethylsulfoxide
-
 Mus musculus
3.3.2.10 NEM
-
 Mus musculus
3.3.2.10 omega-bromo-4-nitroacetophenone
-
 Mus musculus
3.3.2.10 p-Hydroxymercuriphenylsulfonate
-
 Mus musculus
3.3.2.10 tetrahydrofuran
-
 Mus musculus
3.3.2.10 trichloropropylene oxide
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.10 0.0042
-
 trans-stilbene oxide enzyme from clofibrate-treated animals Mus musculus
3.3.2.10 0.0049
-
 trans-stilbene oxide enzyme from untreated animals Mus musculus
3.3.2.10 0.066
-
 cis-stilbene oxide enzyme from clofibrate-treated animals Mus musculus
3.3.2.10 0.073
-
 cis-stilbene oxide enzyme from untreated animals Mus musculus
3.3.2.10 1.36
-
 styrene oxide enzyme from clofibbrate-treated animals Mus musculus
3.3.2.10 1.98
-
 styrene oxide enzyme from untreated animals Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.3.2.10 cytosol
-
 Mus musculus 5829
-

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.10 Mus musculus
-
 untreated and clofibrate-treated animals
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.3.2.10 sensitive to oxygen Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.3.2.10 liver
-
 Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
3.3.2.10 -20°C, stable for at least 6 months Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.10 cis-stilbene oxide + H2O
-
 Mus musculus ?
-
 ?
3.3.2.10 cyclohexene oxide + H2O
-
 Mus musculus ?
-
 ?
3.3.2.10 styrene oxide + H2O
-
 Mus musculus styrene glycol
-
 ?
3.3.2.10 trans-beta-propylstyrene oxide + H2O
-
 Mus musculus ?
-
 ?
3.3.2.10 trans-ethyl styrene oxide + H2O
-
 Mus musculus ?
-
 ?
3.3.2.10 trans-stilbene oxide + H2O
-
 Mus musculus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.10 50
-
-
 Mus musculus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.3.2.10 37 55 37°C: about 60% of maximal activity, 55°C: about 50% of maximal activity of the enzyme from clofibrate-treated animals, about 80% of maximal activity of the enzyme from untreated animals Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.10 6.5 7.4
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.3.2.10 additional information
-
-
 Mus musculus