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Literature summary extracted from

  • von Figura, K.
    Human alpha-N-acetylglucosaminidase. 1. Purification and properties (1977), Eur. J. Biochem., 80, 525-533.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.50 bovine serum albumin stabilization and acceleration of activity betweeen 0.05-0.1%, 2-3fold activity at 0.025% Homo sapiens

General Stability

EC Number General Stability Organism
3.2.1.50 no proteolysis with pronase, bromelain and trypsin, 15% loss of activity after 30 min incubation with papain Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.50 dermatan sulfate competitive inhibition at 1 mM with p-nitrophenyl-alpha-N-acetylglucosaminide and UDP-N-acetylglucosamine as substrates Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.50 0.39
-
UDP-N-acetylglucosamine 0.58 in the presence of 0.025% bovine serum albumin Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.50 Cu2+ complete inhibition at 10 mM Homo sapiens
3.2.1.50 Hg2+ complete inhibition at 10 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.50 300000 307000 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.50 (GlcNAcalpha(1-4))n + H2O Homo sapiens
-
(GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.50 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.50
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.50 urine
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.50 0.115 1.2 purified enzyme, p-nitrophenyl-N-acetyl-alpha-D-glucosaminide as substrate Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.50 (GlcNAcalpha(1-4))n + H2O
-
Homo sapiens (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
?
3.2.1.50 o-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
Homo sapiens o-nitrophenol + N-acetyl-alpha-D-glucosamine
-
?
3.2.1.50 p-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
Homo sapiens p-nitrophenol + N-acetyl-alpha-D-glucosamine
-
?
3.2.1.50 phenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
Homo sapiens phenol + N-acetyl-alpha-D-glucosamine
-
?
3.2.1.50 UDP-N-acetyl-alpha-D-glucosamine + H2O
-
Homo sapiens uridine-5'-diphosphate + N-acetyl-alpha-D-glucosamine
-
?
3.2.1.50 uridine-5'-diphospho-N-acetyl-alpha-D-glucosamine
-
Homo sapiens uridine-5'-diphosphate + N-acetyl-alpha-D-glucosamine
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.50 50 70 90% loss of activity after heating at 60°C for 10 min in the presence of 0.5% bovine serum albumin Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.50 4.2
-
UDP-N-acetylglucosamine Homo sapiens
3.2.1.50 4.6
-
phenyl-alpha-N-acetylglucosaminide Homo sapiens
3.2.1.50 4.8
-
p-nitrophenyl-alpha-N-acetylglucosaminide Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.50 5 9
-
Homo sapiens