EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.50 | bovine serum albumin | stabilization and acceleration of activity betweeen 0.05-0.1%, 2-3fold activity at 0.025% | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.50 | 0.043 | 0.61 | o-nitrophenyl-alpha-N-acetylglucosaminide | 0.23-0.25 in the presence of 0.025% bovine serum albumin | Homo sapiens | |
3.2.1.50 | 0.12 | 1.6 | phenyl-alpha-N-acetylglucosaminide | 0.46-0.66 in the presence of 0.025% bovine serum albumin | Homo sapiens | |
3.2.1.50 | 0.13 | 0.2 | p-nitrophenyl-alpha-N-acetylglucosaminide | 0.21-0.55 in the presence of 0.025% bovine serum albumin | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.50 | 81000 | - |
alpha, beta, 1 * 86500 + 1 * 81000, SDS-PAGE | Homo sapiens |
3.2.1.50 | 86500 | - |
alpha, beta, 1 * 86500 + 1 * 81000, SDS-PAGE | Homo sapiens |
3.2.1.50 | 300000 | 307000 | gel filtration | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.50 | (GlcNAcalpha(1-4))n + H2O | Homo sapiens | involved in the degradation of heparin and heparan sulfate | (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.50 | Homo sapiens | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.50 | glycoprotein | 23.4% carbohydrates, e.g. glucose, mannose, galactose | Homo sapiens |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.50 | - |
Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.50 | placenta | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.50 | 0.115 | 1.2 | purified enzyme, p-nitrophenyl-N-acetyl-alpha-D-glucosaminide as substrate | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.50 | (GlcNAcalpha(1-4))n + H2O | involved in the degradation of heparin and heparan sulfate | Homo sapiens | (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | - |
? | |
3.2.1.50 | o-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O | - |
Homo sapiens | o-nitrophenol + N-acetyl-alpha-D-glucosamine | - |
? | |
3.2.1.50 | p-nitrophenyl-N-acetyl-alpha-D-glucosaminide + H2O | - |
Homo sapiens | p-nitrophenol + N-acetyl-alpha-D-glucosamine | - |
? | |
3.2.1.50 | phenyl-N-acetyl-alpha-D-glucosaminide + H2O | - |
Homo sapiens | phenol + N-acetyl-alpha-D-glucosamine | - |
? | |
3.2.1.50 | uridine-5'-diphospho-N-acetyl-alpha-D-glucosamine | - |
Homo sapiens | uridine-5'-diphosphate + N-acetyl-alpha-D-glucosamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.50 | dimer | alpha, beta, 1 * 86500 + 1 * 81000, SDS-PAGE | Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.50 | 50 | 70 | no effect by 50°C heating, 50% inactivation after 12 min at 60°C and 2 min at 70°C | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.50 | 4.4 | - |
p-nitrophenyl-alpha-N-acetylglucosaminide | Homo sapiens |
3.2.1.50 | 4.5 | - |
o-nitrophenyl-alpha-N-acetylglucosaminide | Homo sapiens |
3.2.1.50 | 4.8 | - |
phenyl-alpha-N-acetylglucosaminide | Homo sapiens |
3.2.1.50 | 5.7 | - |
sucrose-stabilized pH-gradient, after treatment with neuraminidase | Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.50 | 4.3 | 6.5 | - |
Homo sapiens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.50 | 5 | 9 | no loss of activity by dialysis against 100 mM NaCl for 16 h, rapid loss of activity below pH 4 and above pH 10 | Homo sapiens |