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Literature summary extracted from

  • Werries, E.; Wollek, E.; Gottschalk, A.; Buddecke, E.
    Separation of N-acetyl-alpha-glucosaminidase and N-acetyl-alpha-galactosaminidase from ox spleen. Cleavage of the O-glycosidic linkage between carbohydrate and polypeptide in ovine and bovine submaxillary glycoprotein by N-acetyl-alpha-galactosaminidase (1969), Eur. J. Biochem., 10, 445-449.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.49 50
-
 phenyl-alpha-N-acetylglucosaminide
-
 Bos taurus
3.2.1.50 0.12 1.6 phenyl-alpha-N-acetylglucosaminide
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.50 (GlcNAcalpha(1-4))n + H2O Bos taurus
-
 (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?
3.2.1.50 UDP-N-acetyl-alpha-D-glucosamine + H2O Bos taurus
-
 uridine-5'-diphosphate + N-acetyl-alpha-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.49 Bos taurus
-
-
-
3.2.1.50 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.49
-
 Bos taurus
3.2.1.50
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.49 spleen
-
 Bos taurus
-
3.2.1.50 epididymis
-
 Bos taurus
-
3.2.1.50 kidney
-
 Bos taurus
-
3.2.1.50 liver
-
 Bos taurus
-
3.2.1.50 spleen
-
 Bos taurus
-
3.2.1.50 testis
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.50 0.00015 0.0003 homogenates of various organes Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.49 phenyl-alpha-N-acetylglucosaminide + H2O
-
 Bos taurus N-acetylgalactosamine + phenol
-
 ?
3.2.1.50 (GlcNAcalpha(1-4))n + H2O
-
 Bos taurus (GlcNAcalpha(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
 ?
3.2.1.50 phenyl-N-acetyl-alpha-D-glucosaminide + H2O
-
 Bos taurus phenol + N-acetyl-alpha-D-glucosamine
-
 ?
3.2.1.50 UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Bos taurus uridine-5'-diphosphate + N-acetyl-alpha-D-glucosamine
-
 ?
3.2.1.50 uridine-5'-diphospho-N-acetyl-alpha-D-glucosamine
-
 Bos taurus uridine-5'-diphosphate + N-acetyl-alpha-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.50 N-acetyl-alpha-glucosaminidase
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.50 4.5
-
 phenyl-alpha-N-acetylglucosaminide Bos taurus