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Literature summary extracted from

  • Pometto, A.L.; Crawford, D.L.
    Whole-cell bioconversion of vanillin to vanillic acid by Streptomyces viridosporus (1983), Appl. Environ. Microbiol., 45, 1582-1585.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.1.2.61 synthesis useful for commercial microbial batch fermentation of vanillin, economical production of vanillic acid instead of chemical oxidation of vanillin Streptomyces viridosporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.67 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O Streptomyces viridosporus
-
 4-hydroxy-3-methoxybenzoate + NADH + H+
-
 r
1.2.1.67 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O Streptomyces viridosporus T7A / ATCC 39115
-
 4-hydroxy-3-methoxybenzoate + NADH + H+
-
 r
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA Streptomyces viridosporus
-
 vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA Streptomyces viridosporus T7A / ATCC 39115
-
 vanillin + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.67 Streptomyces viridosporus
-
-
-
4.1.2.61 Streptomyces viridosporus
-
-
-
4.1.2.61 Streptomyces viridosporus T7A / ATCC 39115
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.67 cell culture grown in yeast extract-vanillin medium Streptomyces viridosporus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.67 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
-
 Streptomyces viridosporus 4-hydroxy-3-methoxybenzoate + NADH + H+
-
 r
1.2.1.67 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
-
 Streptomyces viridosporus T7A / ATCC 39115 4-hydroxy-3-methoxybenzoate + NADH + H+
-
 r
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Streptomyces viridosporus 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Streptomyces viridosporus T7A / ATCC 39115 4-hydroxy-3-methoxybenzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Streptomyces viridosporus vanillin + acetyl-CoA
-
 ?
4.1.2.61 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propionyl-CoA
-
 Streptomyces viridosporus T7A / ATCC 39115 vanillin + acetyl-CoA
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.61 6
-
-
 Streptomyces viridosporus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.61 5.4 8
-
 Streptomyces viridosporus