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Literature summary extracted from

  • Koszalka, G.W.; Krenitsky, T.A.
    Nucleosidases from Leishmania donovani. Pyrimidine ribonucleosidase, purine ribonucleosidase, and a novel purine 2-deoxyribonucleosidase (1979), J. Biol. Chem., 254, 8185-8193.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.1 2'-deoxyadenosine
-
 Leishmania mexicana
3.2.2.1 2'-deoxyinosine
-
 Leishmania mexicana
3.2.2.1 adenosine potent Leishmania mexicana
3.2.2.1 hypoxanthine arabinoside
-
 Leishmania mexicana
3.2.2.1 purine 2'-deoxyribonucleoside
-
 Leishmania mexicana
3.2.2.1 pyrazolo(3,4-d)pyrimidine
-
 Leishmania mexicana
3.2.2.1 Xanthosine moderate Leishmania mexicana
3.2.2.8 4,6-Dihydroxy-1-beta-D-ribofuranosylpyrazolo-(3,4-d) pyrimidine
-
 Leishmania donovani
3.2.2.8 4-Hydroxyl-1-beta-D-ribofuranosylpyrazolo-(3,4-d) pyrimidine
-
 Leishmania donovani
3.2.2.8 4-Methylthio-1-beta-D-ribofuranosylpyrazolo-(3,4-d)pyrimidine
-
 Leishmania donovani
3.2.2.8 4-Thio-1-beta-D-ribofuranosylpyrazolo-(3,4-d)pyrimidine
-
 Leishmania donovani
3.2.2.8 adenosine
-
 Leishmania donovani
3.2.2.8 guanosine
-
 Leishmania donovani
3.2.2.8 additional information poor inhibitors Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.8 0.33
-
 6-mercaptopurine ribonucleoside
-
 Leishmania donovani
3.2.2.8 0.62
-
 Inosine
-
 Leishmania donovani
3.2.2.8 0.78
-
 purine ribonucleoside
-
 Leishmania donovani
3.2.2.8 1.06
-
 uridine
-
 Leishmania donovani
3.2.2.8 1.11
-
 Xanthosine
-
 Leishmania donovani
3.2.2.8 5.5
-
 uridine
-
 Leishmania donovani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.1 205000
-
 gel filtration Leishmania mexicana
3.2.2.8 180000
-
 gel filtration Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.8 N-D-ribosylpyrimidine Leishmania donovani
-
 pyrimidine + D-ribose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.1 Leishmania mexicana
-
-
-
3.2.2.8 Leishmania donovani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.8
-
 Leishmania donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.8 promastigote
-
 Leishmania donovani
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.8 8.5
-
 substrate: uridine Leishmania donovani

Storage Stability

EC Number Storage Stability Organism
3.2.2.1 -20°C, 5% loss of activity after 30 days Leishmania mexicana
3.2.2.8 3°C, cell extract heated for 10 min at 55°C before passage through a DEAE-cellulose column, enzyme thus purified loses 5% of activity after 30 days Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.1 6-mercaptopurine ribonucleoside + H2O
-
 Leishmania mexicana 6-mercaptopurine + ribose
-
 ?
3.2.2.1 adenosine + H2O no activity Leishmania mexicana D-ribose + adenine
-
 ?
3.2.2.1 guanosine + H2O
-
 Leishmania mexicana D-ribose + guanine
-
 ?
3.2.2.1 inosine + H2O
-
 Leishmania mexicana hypoxanthine + D-ribose
-
 ?
3.2.2.1 purine ribonucleoside + H2O
-
 Leishmania mexicana purine + D-ribose
-
 ?
3.2.2.1 xanthosine + H2O no activity Leishmania mexicana D-ribose + xanthine
-
 ?
3.2.2.8 6-mercaptopurine ribonucleoside + H2O
-
 Leishmania donovani 6-mercaptopurine + D-ribose
-
 ?
3.2.2.8 cytidine + H2O
-
 Leishmania donovani cytosine + D-ribose
-
 ?
3.2.2.8 inosine + H2O
-
 Leishmania donovani hypoxanthine + D-ribose
-
 ?
3.2.2.8 additional information not: adenosine and guanosine Leishmania donovani ?
-
 ?
3.2.2.8 N-D-ribosylpyrimidine
-
 Leishmania donovani pyrimidine + D-ribose
-
 ?
3.2.2.8 N-D-ribosylpyrimidine + H2O
-
 Leishmania donovani pyrimidine + D-ribose
-
 ?
3.2.2.8 purine D-ribonucleosides + H2O appreciable substrate activity if a hydroxyl or thio group is present in the 6-position or if a hydroxyl group is present in the 2- and 6-position, preference for oxo versus amino substituents Leishmania donovani purine + D-ribose
-
 ?
3.2.2.8 purine D-ribonucleosides + H2O prefers pyrimidines Leishmania donovani purine + D-ribose
-
 ?
3.2.2.8 uridine + H2O cleaves uridine most efficiantly Leishmania donovani uracil + D-ribose
-
 ?
3.2.2.8 xanthosine + H2O
-
 Leishmania donovani xanthine + D-ribose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.8 55
-
 after 2 min: all of the uridine-cleaving activity and 62% of the inosine-cleaving activity remains Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.1 6.7
-
-
 Leishmania mexicana
3.2.2.8 6.5
-
 substrate: uridine Leishmania donovani