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Literature summary extracted from

  • Kornberg, S.R.; Lehman, I.R.; Bessman, M.J.; Simms, E.S.; Kornberg, A.
    Enzymatic cleavage of deoxyguanosine triphosphate to deoxyguanosine and tripolyphosphate (1957), J. Biol. Chem., 233, 159-162.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.5.1 dGTP competitive inhibitor Escherichia coli
3.1.5.1 GTP competitive inhibitor Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.5.1 0.002 0.005 dGTP
-
 Escherichia coli
3.1.5.1 0.15
-
 GTP
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.5.1 Mg2+ no activity in absence of Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.5.1 dGTP + H2O Escherichia coli
-
 deoxyguanosine + triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.5.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.5.1
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.5.1 stable at -20°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.5.1 dGTP + H2O
-
 Escherichia coli deoxyguanosine + triphosphate
-
 ?
3.1.5.1 dGTP + H2O highly specific for Escherichia coli deoxyguanosine + triphosphate
-
 ?
3.1.5.1 dGTP + H2O no activity on dGDP Escherichia coli deoxyguanosine + triphosphate
-
 ?
3.1.5.1 GTP + H2O at a lower rate than dGTP Escherichia coli guanosine + triphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.5.1 7.5 8.5 70% activity at pH 9.2 Escherichia coli