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Literature summary extracted from
- Enzymatic hydrolysis of sphingolipids. II. Hydrolysis of sphingomyelin by an enzyme from rat brain (1966), J. Biol. Chem., 241, 3731-3737.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | cholate | - |
Rattus norvegicus |  |
| 3.1.4.12 | Cutsum | - |
Rattus norvegicus | |
| 3.1.4.12 | Detergents | activation | Rattus norvegicus | |
| 3.1.4.12 | ethanol | - |
Rattus norvegicus | |
| 3.1.4.12 | taurocholate | - |
Rattus norvegicus | |
| 3.1.4.12 | Triton X-100 | - |
Rattus norvegicus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | ceramide | - |
Rattus norvegicus | |
| 3.1.4.12 | cetyltrimethylammonium bromide | - |
Rattus norvegicus | |
| 3.1.4.12 | fatty acids | - |
Rattus norvegicus | |
| 3.1.4.12 | lecithin | - |
Rattus norvegicus | |
| 3.1.4.12 | phosphatidylinositol | - |
Rattus norvegicus | |
| 3.1.4.12 | phosphatidylserine | - |
Rattus norvegicus | |
| 3.1.4.12 | sphingosine | - |
Rattus norvegicus | |
| 3.1.4.12 | taurocholate | in presence of Triton X-100 | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.12 | 0.13 | - |
sphingomyelin | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | sphingomyelin + H2O | Rattus norvegicus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | 18fold | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | brain | - |
Rattus norvegicus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.4.12 | -20°C, 250 mM sucrose, 20 mM Tris buffer, several weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | sphingomyelin + H2O | D-erythrosphingosine or dihydroxysphingosine configuration of sphingomyelin results in a 3-5fold faster hydrolysis in comparison to a D-threo or L-erythro configuration | Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
ir | |
| 3.1.4.12 | sphingomyelin + H2O | - |
Rattus norvegicus | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 5 | - |
- |
Rattus norvegicus |
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