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Literature summary extracted from

  • Oleson, A.E.; Sasakuma, M.
    S1 nuclease of Aspergillus oryzae: a glycoprotein with an associated nucleatidase activity (1980), Arch. Biochem. Biophys., 204, 361-370.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.30.1 analysis studies of nucleic acid secondary structure Aspergillus oryzae
3.1.30.1 analysis reagent for mapping splice points and ordered regions in RNA Aspergillus oryzae
3.1.30.1 synthesis industrial production of 5'-phosphomononucleotides from yeast RNA Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.1 2-mercaptoethanol with 1 mM stabilization of activity at pH 3.5 and 4.0 Aspergillus oryzae
3.1.30.1 NaCl maximal activity at 0-100 mM, at 1 mM 71% of maximal activity Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.30.1 0.04
-
 3'-AMP
-
 Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.1 Zn2+ 46% stimulation of activity at 0.1 mM Zn2+, maximal stimulation at 0.01 mM Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.1 38000
-
 SDS-PAGE Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.1 DNA + H2O Aspergillus oryzae
-
 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.1 Aspergillus oryzae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.30.1 glycoprotein
-
 Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.1
-
 Aspergillus oryzae

Storage Stability

EC Number Storage Stability Organism
3.1.30.1 4°C, 0.1 M buffer pH 3.5-4.0, mercaptoethanol, 22 h, 16.9% of initial activity at pH 3.5, 59% at pH 4.0, by preincubation with Zn2+ 48% of initial activity at pH 3.5, 86% at pH 4.0 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.1 2'-AMP + H2O
-
 Aspergillus oryzae adenosine + phosphate
-
 ?
3.1.30.1 3'-AMP + H2O
-
 Aspergillus oryzae adenosine + phosphate
-
 ?
3.1.30.1 3'-CMP + H2O
-
 Aspergillus oryzae cytosine + phosphate
-
 ?
3.1.30.1 3'-dAMP + H2O
-
 Aspergillus oryzae 3'-deoxyadenosine + phosphate
-
 ?
3.1.30.1 3'-dTMP + H2O
-
 Aspergillus oryzae deoxythymidine + phosphate
-
 ?
3.1.30.1 3'-GMP + H2O
-
 Aspergillus oryzae guanosine + phosphate
-
 ?
3.1.30.1 3'-UMP + H2O
-
 Aspergillus oryzae uridine + phosphate
-
 ?
3.1.30.1 adenosine 3',5'-bisphosphate + H2O
-
 Aspergillus oryzae 5'-AMP + phosphate
-
 ?
3.1.30.1 DNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?
3.1.30.1 ssDNA + H2O
-
 Aspergillus oryzae 5'-phosphomononucleotides + 5'-phosphooligonucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.30.1 S1 nuclease
-
 Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.1 6 7 with 3'-AMP and adenosine 3',5'-biphosphate as substrate Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.30.1 3 8.5 with mononucleotide substrates Aspergillus oryzae