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Literature summary extracted from

  • Dangel, W.; Tschech, A.; Fuchs, G.
    Enzyme reactions involved in anaerobic cyclohexanol metabolism by a denitrifying Pseudomonas species (1989), Arch. Microbiol., 152, 273-279.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.99.14 2-cyclohexenone
-
Pseudomonas sp.
3.7.1.10 1,2-Cyclohexanedione
-
Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.245 0.04
-
NAD+
-
Pseudomonas sp.
1.1.1.245 0.05
-
Cyclohexanol
-
Pseudomonas sp.
3.7.1.10 0.07
-
Cyclohexane-1,3-dione
-
Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.245 cyclohexanol + NAD+ Pseudomonas sp. inducible cyclohexanone + NADH
-
?
1.1.1.245 cyclohexanol + NAD+ Pseudomonas sp. metabolism of cyclohexane cyclohexanone + NADH
-
?
1.3.99.14 Cyclohexanone + acceptor Pseudomonas sp. the enzyme is involved in anaerobic degradation of cyclohexanol ?
-
?
3.7.1.10 cyclohexane-1,3-dione + H2O Pseudomonas sp. enzyme of anaerobic cyclohexanol metabolism ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.245 Pseudomonas sp.
-
-
-
1.1.99.26 Pseudomonas sp.
-
enzyme is induced by growth on cyclohexanone under anaerobic conditions
-
1.3.99.14 Pseudomonas sp.
-
denitrifying species
-
3.7.1.10 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.26
-
Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.99.14 culture condition:cyclohexanol-grown cell anaerobically or aerobically Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.245 0.8
-
oxidation of cyclohexanol at pH 7.8 Pseudomonas sp.
1.1.1.245 1.9
-
reduction of cyclohexanone at pH 7.8 Pseudomonas sp.
1.1.1.245 2.5
-
pH 10 Pseudomonas sp.
1.1.99.26 0.27
-
2-cyclohexenone hydratase and 3-hydroxycyclohexanone dehydrogenase activity in cell extracts Pseudomonas sp.
1.3.99.14 0.7
-
-
Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.245 -20°C, stable for several days Pseudomonas sp.
1.1.99.26 -20°C, cell extract stable for several days Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.245 1,2-cycloheptanediol + NAD+ relative oxidation activity at 25% the rate of cyclohexanol oxidation Pseudomonas sp. cycloheptane-1,2-dione + NADH
-
?
1.1.1.245 1-heptanol + NAD+ relative oxidation activity at 32% the rate of cyclohexanol oxidation Pseudomonas sp. heptanal + NADH
-
?
1.1.1.245 1-hexanol + NAD+ relative oxidation activity at 7% the rate of cyclohexanol oxidation Pseudomonas sp. hexanal + NADH
-
?
1.1.1.245 2-cyclohexanol + NAD+ relative oxidation activity at 142% the rate of cyclohexanol oxidation Pseudomonas sp. 2-cyclohexenone + NADH slow reduction r
1.1.1.245 2-hydroxycyclohexanone + NADH relative activity at 50% the rate of cyclohexanone reduction Pseudomonas sp. ? + NAD+
-
?
1.1.1.245 cycloheptanone + NADH relative activity at 10% the rate of cyclohexanone reduction Pseudomonas sp. cycloheptanol + NAD+
-
?
1.1.1.245 cyclohexan-1,2-diol + NAD+ relative oxidation activity at 15% the rate of cyclohexanol oxidation Pseudomonas sp. cyclohexane-1,2-dione + NADH slow reduction r
1.1.1.245 cyclohexane-1,3-diol + NAD+ relative oxidation activity at 14% the rate of cyclohexanol oxidation Pseudomonas sp. cyclohexane-1,3-dione + NADH slow reduction r
1.1.1.245 cyclohexane-1,4-diol + NAD+ relative oxidation activity at 36% the rate of cyclohexanol oxidation Pseudomonas sp. cyclohexane-1,4-dione + NADH relative reduction activity at 240% the rate of cyclohexanone reduction r
1.1.1.245 cyclohexanol + NAD+
-
Pseudomonas sp. cyclohexanone + NADH
-
r
1.1.1.245 cyclohexanol + NAD+ inducible Pseudomonas sp. cyclohexanone + NADH
-
?
1.1.1.245 cyclohexanol + NAD+ metabolism of cyclohexane Pseudomonas sp. cyclohexanone + NADH
-
?
1.1.1.245 cyclopentanol + NAD+
-
Pseudomonas sp. cyclopentanone + NADH relative reduction activity at 4% the rate of cyclohexanone reduction r
1.1.1.245 additional information 2-hydroxycyclohexanone, 1,3,5-trihydroxycyclohexane, menthol, ethanol, 1-propanol, 1-butanol and 1-pentanol are not oxidized with NAD+ Pseudomonas sp. additional information 2-cyclopentenone, 1,3-cyclohexanedione, 1,3-cyclopentanedione and 5-oxocapronate are not reduced with NADH ?
1.1.99.26 3-hydroxycyclohexanone + acceptor methylene blue or 2,6-dichlorophenolindophenol can function as electron acceptor in vitro Pseudomonas sp. 1,3-cyclohexanedione + reduced acceptor
-
?
1.3.99.14 cyclohex-2-enone + 2,6-dichlorophenol indophenol about 30% of the activity with cyclohexanone Pseudomonas sp. phenol + reduced 2,6-dichlorophenol indophenol
-
?
1.3.99.14 cyclohexanone + 2,6-dichlorophenol indophenol no activity as acceptor: NAD+, NADP+, methylene blue Pseudomonas sp. cyclohex-2-enone + reduced 2,6-dichlorophenol indophenol
-
?
1.3.99.14 Cyclohexanone + acceptor the enzyme is involved in anaerobic degradation of cyclohexanol Pseudomonas sp. ?
-
?
3.7.1.10 cyclohexane-1,3-dione + H2O highly specific, does not act on other dione derivatives of cyclohexane, cyclopentane or cycloheptane Pseudomonas sp. 5-oxohexanoate
-
?
3.7.1.10 cyclohexane-1,3-dione + H2O enzyme of anaerobic cyclohexanol metabolism Pseudomonas sp. ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.26 30
-
assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.245 10
-
-
Pseudomonas sp.
1.1.99.26 7.8
-
assay at Pseudomonas sp.
1.3.99.14 9.6
-
-
Pseudomonas sp.
3.7.1.10 7.1
-
-
Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.245 10.5
-
rapid inactivation above pH 10.5 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.245 NAD+
-
Pseudomonas sp.
1.1.1.245 NADP+ less than 5% activity than with NAD+ Pseudomonas sp.