EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.99.14 | 2-cyclohexenone | - |
Pseudomonas sp. | |
3.7.1.10 | 1,2-Cyclohexanedione | - |
Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.245 | 0.04 | - |
NAD+ | - |
Pseudomonas sp. | |
1.1.1.245 | 0.05 | - |
Cyclohexanol | - |
Pseudomonas sp. | |
3.7.1.10 | 0.07 | - |
Cyclohexane-1,3-dione | - |
Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.245 | cyclohexanol + NAD+ | Pseudomonas sp. | inducible | cyclohexanone + NADH | - |
? | |
1.1.1.245 | cyclohexanol + NAD+ | Pseudomonas sp. | metabolism of cyclohexane | cyclohexanone + NADH | - |
? | |
1.3.99.14 | Cyclohexanone + acceptor | Pseudomonas sp. | the enzyme is involved in anaerobic degradation of cyclohexanol | ? | - |
? | |
3.7.1.10 | cyclohexane-1,3-dione + H2O | Pseudomonas sp. | enzyme of anaerobic cyclohexanol metabolism | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.245 | Pseudomonas sp. | - |
- |
- |
1.1.99.26 | Pseudomonas sp. | - |
enzyme is induced by growth on cyclohexanone under anaerobic conditions | - |
1.3.99.14 | Pseudomonas sp. | - |
denitrifying species | - |
3.7.1.10 | Pseudomonas sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.26 | - |
Pseudomonas sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.99.14 | culture condition:cyclohexanol-grown cell | anaerobically or aerobically | Pseudomonas sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.245 | 0.8 | - |
oxidation of cyclohexanol at pH 7.8 | Pseudomonas sp. |
1.1.1.245 | 1.9 | - |
reduction of cyclohexanone at pH 7.8 | Pseudomonas sp. |
1.1.1.245 | 2.5 | - |
pH 10 | Pseudomonas sp. |
1.1.99.26 | 0.27 | - |
2-cyclohexenone hydratase and 3-hydroxycyclohexanone dehydrogenase activity in cell extracts | Pseudomonas sp. |
1.3.99.14 | 0.7 | - |
- |
Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.245 | -20°C, stable for several days | Pseudomonas sp. |
1.1.99.26 | -20°C, cell extract stable for several days | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.245 | 1,2-cycloheptanediol + NAD+ | relative oxidation activity at 25% the rate of cyclohexanol oxidation | Pseudomonas sp. | cycloheptane-1,2-dione + NADH | - |
? | |
1.1.1.245 | 1-heptanol + NAD+ | relative oxidation activity at 32% the rate of cyclohexanol oxidation | Pseudomonas sp. | heptanal + NADH | - |
? | |
1.1.1.245 | 1-hexanol + NAD+ | relative oxidation activity at 7% the rate of cyclohexanol oxidation | Pseudomonas sp. | hexanal + NADH | - |
? | |
1.1.1.245 | 2-cyclohexanol + NAD+ | relative oxidation activity at 142% the rate of cyclohexanol oxidation | Pseudomonas sp. | 2-cyclohexenone + NADH | slow reduction | r | |
1.1.1.245 | 2-hydroxycyclohexanone + NADH | relative activity at 50% the rate of cyclohexanone reduction | Pseudomonas sp. | ? + NAD+ | - |
? | |
1.1.1.245 | cycloheptanone + NADH | relative activity at 10% the rate of cyclohexanone reduction | Pseudomonas sp. | cycloheptanol + NAD+ | - |
? | |
1.1.1.245 | cyclohexan-1,2-diol + NAD+ | relative oxidation activity at 15% the rate of cyclohexanol oxidation | Pseudomonas sp. | cyclohexane-1,2-dione + NADH | slow reduction | r | |
1.1.1.245 | cyclohexane-1,3-diol + NAD+ | relative oxidation activity at 14% the rate of cyclohexanol oxidation | Pseudomonas sp. | cyclohexane-1,3-dione + NADH | slow reduction | r | |
1.1.1.245 | cyclohexane-1,4-diol + NAD+ | relative oxidation activity at 36% the rate of cyclohexanol oxidation | Pseudomonas sp. | cyclohexane-1,4-dione + NADH | relative reduction activity at 240% the rate of cyclohexanone reduction | r | |
1.1.1.245 | cyclohexanol + NAD+ | - |
Pseudomonas sp. | cyclohexanone + NADH | - |
r | |
1.1.1.245 | cyclohexanol + NAD+ | inducible | Pseudomonas sp. | cyclohexanone + NADH | - |
? | |
1.1.1.245 | cyclohexanol + NAD+ | metabolism of cyclohexane | Pseudomonas sp. | cyclohexanone + NADH | - |
? | |
1.1.1.245 | cyclopentanol + NAD+ | - |
Pseudomonas sp. | cyclopentanone + NADH | relative reduction activity at 4% the rate of cyclohexanone reduction | r | |
1.1.1.245 | additional information | 2-hydroxycyclohexanone, 1,3,5-trihydroxycyclohexane, menthol, ethanol, 1-propanol, 1-butanol and 1-pentanol are not oxidized with NAD+ | Pseudomonas sp. | additional information | 2-cyclopentenone, 1,3-cyclohexanedione, 1,3-cyclopentanedione and 5-oxocapronate are not reduced with NADH | ? | |
1.1.99.26 | 3-hydroxycyclohexanone + acceptor | methylene blue or 2,6-dichlorophenolindophenol can function as electron acceptor in vitro | Pseudomonas sp. | 1,3-cyclohexanedione + reduced acceptor | - |
? | |
1.3.99.14 | cyclohex-2-enone + 2,6-dichlorophenol indophenol | about 30% of the activity with cyclohexanone | Pseudomonas sp. | phenol + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.3.99.14 | cyclohexanone + 2,6-dichlorophenol indophenol | no activity as acceptor: NAD+, NADP+, methylene blue | Pseudomonas sp. | cyclohex-2-enone + reduced 2,6-dichlorophenol indophenol | - |
? | |
1.3.99.14 | Cyclohexanone + acceptor | the enzyme is involved in anaerobic degradation of cyclohexanol | Pseudomonas sp. | ? | - |
? | |
3.7.1.10 | cyclohexane-1,3-dione + H2O | highly specific, does not act on other dione derivatives of cyclohexane, cyclopentane or cycloheptane | Pseudomonas sp. | 5-oxohexanoate | - |
? | |
3.7.1.10 | cyclohexane-1,3-dione + H2O | enzyme of anaerobic cyclohexanol metabolism | Pseudomonas sp. | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.26 | 30 | - |
assay at | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.245 | 10 | - |
- |
Pseudomonas sp. |
1.1.99.26 | 7.8 | - |
assay at | Pseudomonas sp. |
1.3.99.14 | 9.6 | - |
- |
Pseudomonas sp. |
3.7.1.10 | 7.1 | - |
- |
Pseudomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.245 | 10.5 | - |
rapid inactivation above pH 10.5 | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.245 | NAD+ | - |
Pseudomonas sp. | |
1.1.1.245 | NADP+ | less than 5% activity than with NAD+ | Pseudomonas sp. |