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Literature summary extracted from

  • De Jong, E.; van Berkel, W.J.H.; van der Zwan, R.P.; de Bont, J.A.M.
    Purification and characterization of vanillyl-alcohol oxidase from Penicillium simplicissimum. A novel aromatic alcohol oxidase containing covalently bound FAD (1992), Eur. J. Biochem., 208, 651-657.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.38 4-hydroxyphenylethyl alcohol
-
 Penicillium simplicissimum
1.1.3.38 cinnamyl alcohol strong competitive inhibitor of vanillyl-alcohol oxidation Penicillium simplicissimum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.38 0.012
-
 O2
-
 Penicillium simplicissimum
1.1.3.38 0.043
-
 phenazine methosulfate
-
 Penicillium simplicissimum
1.1.3.38 0.73
-
 Vanillyl alcohol
-
 Penicillium simplicissimum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.38 65000
-
 8 * 65000, SDS-PAGE Penicillium simplicissimum
1.1.3.38 520000
-
 gel filtration Penicillium simplicissimum

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.38 Penicillium simplicissimum
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.3.38 photoreduction of the enzyme in the presence of the competitive inhibitor cinnamyl alcohol gives rise to a complete irreversible bleaching of the flavin spectrum Penicillium simplicissimum

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.38
-
 Penicillium simplicissimum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.38 culture condition:veratryl alcohol-grown cell sole source of carbon and energy Penicillium simplicissimum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.38 2.13
-
-
 Penicillium simplicissimum

Storage Stability

EC Number Storage Stability Organism
1.1.3.38 -20°C or 4°C, in 20% (NH4)2SO4, pH 7.0, stable for several weeks Penicillium simplicissimum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.38 4-hydroxybenzyl alcohol + O2 at 75% of the activity with vanillyl alcohol Penicillium simplicissimum 4-hydroxybenzaldehyde + H2O2
-
 ?
1.1.3.38 vanillyl alcohol + O2
-
 Penicillium simplicissimum vanillin + H2O2
-
 ?
1.1.3.38 vanillyl alcohol + phenazine methosulfate
-
 Penicillium simplicissimum vanillin + reduced phenazine methosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.38 octamer 8 * 65000, SDS-PAGE Penicillium simplicissimum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.38 38
-
-
 Penicillium simplicissimum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.38 45
-
 90 min, stable Penicillium simplicissimum
1.1.3.38 60
-
 45 min, 50% loss of activity Penicillium simplicissimum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.38 9 10.5
-
 Penicillium simplicissimum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.38 7 12 about 30% of maximal activity at pH 7 and pH 12 Penicillium simplicissimum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.38 8alpha-(N3-Histidyl)-FAD the octamer contains 1 mol of covalently-bound flavin per mol of subunit Penicillium simplicissimum