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Literature summary for 7.4.2.8 extracted from

  • Imada, K.; Minamino, T.; Uchida, Y.; Kinoshita, M.; Namba, K.
    Insight into the flagella type III export revealed by the complex structure of the type III ATPase and its regulator (2016), Proc. Natl. Acad. Sci. USA, 113, 3633-3638 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Salmonella enterica

Crystallization (Commentary)

Crystallization (Comment) Organism
FliHC2-FliI complex, sitting drop vapor diffusion method, using 0.1 M HEPES-NaOH (pH 7.2), 5% (w/v) PEG-400, and 0.1 M magnesium acetate Salmonella enterica

Protein Variants

Protein Variants Comment Organism
E16A the mutation slightly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
F15A the mutation slightly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
L12A the mutation significantly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
R26A/R27A/R33A the mutations significantly reduce the binding affinity of the ATPase FliI for FliH Salmonella enterica
R4A the mutation significantly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
R4A/R7A the mutation significantly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
R7A the mutation slightly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
W8A the mutation significantly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Salmonella enterica 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + cellular protein[side 1] Salmonella enterica
-
 ADP + phosphate + cellular protein[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
 serovar typhimurium
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + cellular protein[side 1]
-
 Salmonella enterica ADP + phosphate + cellular protein[side 2]
-
 ?

Subunits

Subunits Comment Organism
homohexamer
-
 Salmonella enterica

Synonyms

Synonyms Comment Organism
FliI Walker-type ATPase component of the FliI6FliJ ATPase complex Salmonella enterica
FliI6FliJ complex FliI and FliJ form the FliI6FliJ ATPase complex of the bacterial flagellar export apparatus Salmonella enterica