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Literature summary for 7.4.2.8 extracted from

  • Lorenz, C.; Hausner, J.; Buettner, D.
    HrcQ provides a docking site for early and late type III secretion substrates from Xanthomonas (2012), PLoS ONE, 7, e51063.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.8

Cloned(Commentary)

Cloned (Comment) Organism
expression of C-terminally c-Myc-tagged ATPase HrcN under control of the lac promoter into Xanthomonas campestris strains 85-10DhrcQ and 85*DhrcQ, co-expression with GST-HrcQ and C-terminally c-Myc-tagged HrcL in Escherichia coli Xanthomonas campestris

Protein Variants

Protein Variants Comment Organism
additional information GST fusions of the cytoplasmic T3S ATPase HrcN and its predicted regulator HrcL are immobilized on glutathione sepharose and incubated with a bacterial lysate containing HrcQ-c-Myc, binding and interaction analysis, overview Xanthomonas campestris

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm the enzyme binds to the membrane-located type III secretion system complex Xanthomonas campestris 5737
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Xanthomonas campestris
-
 ADP + phosphate
-
 ?
ATP + H2O Xanthomonas campestris 85-10
-
 ADP + phosphate
-
 ?
additional information Xanthomonas campestris enzyme ATPase HrcN interacts with its regulator HrcL and substrate acceptor site HrcQ ?
-
 ?
additional information Xanthomonas campestris 85-10 enzyme ATPase HrcN interacts with its regulator HrcL and substrate acceptor site HrcQ ?
-
 ?

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
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 pv. vesicatoria
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Xanthomonas campestris 85-10
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 pv. vesicatoria
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Xanthomonas campestris ADP + phosphate
-
 ?
ATP + H2O
-
 Xanthomonas campestris 85-10 ADP + phosphate
-
 ?
additional information enzyme ATPase HrcN interacts with its regulator HrcL and substrate acceptor site HrcQ Xanthomonas campestris ?
-
 ?
additional information enzyme ATPase HrcN interacts with its regulator HrcL and substrate acceptor site HrcQ Xanthomonas campestris 85-10 ?
-
 ?

Synonyms

Synonyms Comment Organism
HrcN
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 Xanthomonas campestris
T3S-ATPase
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 Xanthomonas campestris

General Information

General Information Comment Organism
metabolism pathogenicity of many Gram-negative bacteria depends on a type III secretion (T3S) system which translocates bacterial effector proteins into eukaryotic cells. The membrane-spanning secretion apparatus is associated with a cytoplasmic ATPase complex and a predicted cytoplasmic (C) ring structure which is proposed to provide a substrate docking platform for secreted proteins. The putative C ring component HrcQ from the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria is essential for bacterial pathogenicity and type III secretion system, overview. The general substrate acceptor site of the T3S system, HrcQ, localizes to the cytoplasm and associates with the bacterial membranes under type III secretion system-permissive conditions binding to the cytoplasmic T3S-ATPase HrcN, its predicted regulator HrcL and the cytoplasmic domains of the inner membrane proteins HrcV and HrcU. HpaB presumably targets effector proteins to the ATPase HrcN of the T3S system which can dissociate HpaB-effector protein complexes and thus might facilitate the entry of effector proteins into the inner channel of the T3S system Xanthomonas campestris
physiological function HrcQ interacts with conserved components of the type-III secretion system at the inner membrane including the inner membrane proteins HrcV and HrcU, the ATPase HrcN and its predicted regulator HrcL Xanthomonas campestris