Literature summary for 7.4.2.8 extracted from

Lu, C.; Korotkov, K.V.; Hol, W.G.
Crystal structure of the full-length ATPase GspE from the Vibrio vulnificus type II secretion system in complex with the cytoplasmic domain of GspL (2014), J. Struct. Biol., 187, 223-235.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Vibrio vulnificus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme GspE in complex with cyto-GspL, sitting drop vapor diffusion method, by mixing of 0.001 ml of protein in 20 mM HEPES, pH7.5, 200 mM NaCl buffer with 0.001 ml of reservoir solution containing 0.2 M Na malonate pH 7.0, 18% PEG 3350, at 21°C, X-ray diffraction structure determination and analysis at 2.83 A resolution, molecular replacement using the structures of Vibrio cholerae DELTAN1EGspE (PDB ID 1P9R) and the Vibrio cholerae N1E-cyto-GspL complex (PDB ID 2BH1) as search models	Vibrio vulnificus

Crystallization (Comment)

Organism

purified recombinant enzyme GspE in complex with cyto-GspL, sitting drop vapor diffusion method, by mixing of 0.001 ml of protein in 20 mM HEPES, pH7.5, 200 mM NaCl buffer with 0.001 ml of reservoir solution containing 0.2 M Na malonate pH 7.0, 18% PEG 3350, at 21°C, X-ray diffraction structure determination and analysis at 2.83 A resolution, molecular replacement using the structures of Vibrio cholerae DELTAN1EGspE (PDB ID 1P9R) and the Vibrio cholerae N1E-cyto-GspL complex (PDB ID 2BH1) as search models

Vibrio vulnificus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Vibrio vulnificus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
83000	-	3 * 83000, GspE-cyto-GspL complex, crystal structure analysis	Vibrio vulnificus
250000	-	GspE-cyto-GspL complex trimer, crystal structure analysis	Vibrio vulnificus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Vibrio vulnificus	-	ADP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Vibrio vulnificus	Q7MPZ5	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	Vibrio vulnificus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Vibrio vulnificus	ADP + phosphate	-	?

Subunits

Subunits	Comment	Organism
More	GspE is a protein of about 500 residues folding into three major domains, the N-terminal domains N1E and N2E, and the C-terminal domain CTE. The N2E and CTE domains of a single GspE subunit adopt a mutual orientation	Vibrio vulnificus
trimer	3 * 83000, GspE-cyto-GspL complex, crystal structure analysis	Vibrio vulnificus

Synonyms

Synonyms	Comment	Organism
EpsE	-	Vibrio vulnificus
GspE	-	Vibrio vulnificus
secretion ATPase	-	Vibrio vulnificus
T2SS secretion ATPase	-	Vibrio vulnificus
type II secretory pathway ATPase	-	Vibrio vulnificus

General Information

General Information	Comment	Organism
metabolism	the enzyme is part of the type II secretion system (T2SS), which is present in many Gram-negative bacteria and is responsible for secreting a large number of folded proteins, including major virulence factors, across the outer membrane	Vibrio vulnificus
physiological function	among the many protein components of the T2SS, the secretion ATPase GspE plays an essential role, and is likely responsible for providing energy for the protein translocation process	Vibrio vulnificus