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Literature summary for 7.4.2.8 extracted from

  • Chen, Y.; Zhang, X.; Manias, D.; Yeo, H.; Dunny, G.M.; Christie, P.J.
    Enterococcus faecalis PcfC, a spatially localized substrate receptor for type IV secretion of the pCF10 transfer intermediate (2008), J. Bacteriol., 190, 3632-3645.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.8

Cloned(Commentary)

Cloned (Comment) Organism
nine pcfF expression plasmids are constructed, different in the combination of the promotor, Pnis, P23, Ptac, PT7, the affinity tag, His6, GST, and the cloned PcfC fragment, complete cDNA, deltaN103 or mutant K156T Enterococcus faecalis

Protein Variants

Protein Variants Comment Organism
deltaN103 pcfF mutant lacking codon 1 to 103 Enterococcus faecalis
K156T pcfF mutant Enterococcus faecalis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Enterococcus faecalis 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 His6-PcfCdeltaN103, determined by size exclusion chromatography Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Enterococcus faecalis in addition the protein binds DNA and interacts with PcfF and PcfG ADP + phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography, using Talon, Co2+, resin and glutathione-Sepharose beads, and by gel filtration Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O in addition the protein binds DNA and interacts with PcfF and PcfG Enterococcus faecalis ADP + phosphate
-
 ir

Synonyms

Synonyms Comment Organism
PcfC
-
 Enterococcus faecalis
protein-secreting ATPase
-
 Enterococcus faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 ATP binding assay at room temperature Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 ATP binding assay Enterococcus faecalis