Literature summary for 7.4.2.8 extracted from

Blaylock, B.; Riordan, K.E.; Missiakas, D.M.; Schneewind, O.
Characterization of the Yersinia enterocolitica type III secretion ATPase YscN and its regulator, YscL (2006), J. Bacteriol., 188, 3525-3534.

Search for more literature for 7.4.2.8

Application

Application	Comment	Organism
biotechnology	glutathione S-transferase-enzyme fusion protein functions analogously to native protein. Overexpression of regulatory protein YscL impairs secretion	Yersinia enterocolitica

Protein Variants

Protein Variants	Comment	Organism
K175E	mutation in one of hte Walker boxes, enzymatically inactive	Yersinia enterocolitica

Inhibitors

Inhibitors	Comment	Organism	Structure
YscN	intrinsic regulatory protein, noncompetitive inhibition by allosteric binding	Yersinia enterocolitica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	enzyme shows positive cooperativity and non-Michaelis-Menten kinetics	Yersinia enterocolitica

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	stimulation of sectretion	Yersinia enterocolitica

Organism

Organism	UniProt	Comment	Textmining
Yersinia enterocolitica	-	type III secretion ATPase YscN	-

Subunits

Subunits	Comment	Organism
More	upon addition of ATP, purified enzyme forms aggregates, probably hexamers	Yersinia enterocolitica

Synonyms

Synonyms	Comment	Organism
YscN	-	Yersinia enterocolitica

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Release 2023.1 (January 2023)