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Literature summary for 7.4.2.8 extracted from

  • Schoenhofen, I.C.; Li, G.; Strozen, T.G.; Howard, S.P.
    Purification and characterization of the N-terminal domain of ExeA: A novel ATPase involved in the type II secretion pathway of Aeromonas hydrophila (2005), J. Bacteriol., 187, 6370-6378.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.4.2.8

Protein Variants

Protein Variants Comment Organism
additional information expression of N-terminal cytoplasmic domain, domain shows ATPase activity Aeromonas hydrophila

Inhibitors

Inhibitors Comment Organism Structure
1,3-dicyclohexylcarbodiimide 0.01 mM, 76% residual activity Aeromonas hydrophila
5'-adenylylimidodiphosphate 1 mM, 12% residual activity Aeromonas hydrophila
KNO3 10 mM, 74% residual activity Aeromonas hydrophila
Sodium azide 10 mM, 10% residual activity Aeromonas hydrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 MgATP pH 7.5, 37°C, ATPase activity of N-terminal cytoplasmic domain Aeromonas hydrophila

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ preferred cation, N-terminal cytoplasmic domain Aeromonas hydrophila

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila
-
 type II secretory pathway
-

Purification (Commentary)

Purification (Comment) Organism
N-terminal cytoplasmic domain, with His-tag Aeromonas hydrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
MgATP + H2O
-
 Aeromonas hydrophila MgADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
More enzyme associates with intrinsic protein exeB to forms large complexes Aeromonas hydrophila

Synonyms

Synonyms Comment Organism
ExeA
-
 Aeromonas hydrophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 42 ATPase activity of N-terminal cytoplasmic domain Aeromonas hydrophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 ATPase activity of N-terminal cytoplasmic domain Aeromonas hydrophila