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Literature summary for 6.6.1.1 extracted from

  • Adams, N.B.; Marklew, C.J.; Brindley, A.A.; Hunter, C.N.; Reid, J.D.
    Characterization of the magnesium chelatase from Thermosynechococcus elongatus (2014), Biochem. J., 457, 163-170.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Synechocystis sp.
expressed in Escherichia coli as a His-tagged fusion protein Thermosynechococcus vestitus

Protein Variants

Protein Variants Comment Organism
additional information Synechocystis chelatase tolerates substitution of individual subunits with their Thermosynechococcus equivalents to produce hybrid enzymes.The heterologous complex is much less active than either of the two parent enzymes Synechocystis sp.
additional information Synechocystis chelatase tolerates substitution of individual subunits with their Thermosynechococcus equivalents to produce hybrid enzymes.The heterologous complex is much less active than either of the two parent enzymes Thermosynechococcus vestitus

Inhibitors

Inhibitors Comment Organism Structure
porphyrin at high ChlH concentrations, substrate inhibition is very noticeable Thermosynechococcus vestitus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
ATP pH 7.7, 45°C Thermosynechococcus vestitus
2.3
-
Mg2+ pH 7.7, 45°C Thermosynechococcus vestitus

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-
Thermosynechococcus vestitus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Synechocystis sp.
using Ni-NTA chromatography Thermosynechococcus vestitus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protoporphyrin IX + Mg2+ + H2O
-
Thermosynechococcus vestitus ADP + phosphate + Mg-protoporphyrin IX + H+
-
?

Synonyms

Synonyms Comment Organism
chelatase H
-
Thermosynechococcus vestitus
magnesium chelatase
-
Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Thermosynechococcus vestitus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
melting temperature Thermosynechococcus vestitus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0061
-
ATP pH 7.7, 45°C Thermosynechococcus vestitus
0.0065
-
Mg2+ pH 7.7, 45°C Thermosynechococcus vestitus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
assay at Thermosynechococcus vestitus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.005
-
porphyrin pH 7.7, 45°C. At high ChlH concentrations, substrate inhibition is very noticeable Thermosynechococcus vestitus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0028
-
Mg2+ pH 7.7, 45°C Thermosynechococcus vestitus
0.0075
-
ATP pH 7.7, 45°C Thermosynechococcus vestitus