Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Synechocystis sp. |
expressed in Escherichia coli as a His-tagged fusion protein | Thermosynechococcus vestitus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Synechocystis chelatase tolerates substitution of individual subunits with their Thermosynechococcus equivalents to produce hybrid enzymes.The heterologous complex is much less active than either of the two parent enzymes | Synechocystis sp. |
additional information | Synechocystis chelatase tolerates substitution of individual subunits with their Thermosynechococcus equivalents to produce hybrid enzymes.The heterologous complex is much less active than either of the two parent enzymes | Thermosynechococcus vestitus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
porphyrin | at high ChlH concentrations, substrate inhibition is very noticeable | Thermosynechococcus vestitus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
ATP | pH 7.7, 45°C | Thermosynechococcus vestitus | |
2.3 | - |
Mg2+ | pH 7.7, 45°C | Thermosynechococcus vestitus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Thermosynechococcus vestitus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Synechocystis sp. |
using Ni-NTA chromatography | Thermosynechococcus vestitus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protoporphyrin IX + Mg2+ + H2O | - |
Thermosynechococcus vestitus | ADP + phosphate + Mg-protoporphyrin IX + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chelatase H | - |
Thermosynechococcus vestitus |
magnesium chelatase | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Thermosynechococcus vestitus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
melting temperature | Thermosynechococcus vestitus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
ATP | pH 7.7, 45°C | Thermosynechococcus vestitus | |
0.0065 | - |
Mg2+ | pH 7.7, 45°C | Thermosynechococcus vestitus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Thermosynechococcus vestitus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
porphyrin | pH 7.7, 45°C. At high ChlH concentrations, substrate inhibition is very noticeable | Thermosynechococcus vestitus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
Mg2+ | pH 7.7, 45°C | Thermosynechococcus vestitus | |
0.0075 | - |
ATP | pH 7.7, 45°C | Thermosynechococcus vestitus |