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Literature summary for 6.6.1.1 extracted from

  • Tsuzuki, T.; Takahashi, K.; Inoue, S.; Okigaki, Y.; Tomiyama, M.; Hossain, M.; Shimazaki, K.; Murata, Y.; Kinoshita, T.
    Mg-chelatase H subunit affects ABA signaling in stomatal guard cells, but is not an ABA receptor in Arabidopsis thaliana (2011), J. Plant Res., 124, 527-538.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
L690F missense mutation of CHLH responsible for the rtl1 phenotype with abscisic acid-insensitive stomatal movements Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
ecotype Columbia
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and glutathione Sepharose 4B bead chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
guard cell
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protoporphyrin IX + Mg2+ + H2O
-
Arabidopsis thaliana ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
additional information CHLH does not bind abscisic acid Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
ChlH subunit Arabidopsis thaliana
Mg-chelatase H subunit Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction CHLH RNAi-silenced plants show phenotypes in which stomatal movements are insensitive to abscisic acid Arabidopsis thaliana
physiological function Mg-chelatase H subunit affects abscisic acid signaling in stomatal guard cells, but is not an abscisic acid receptor Arabidopsis thaliana