Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Gun4 | addition of Gun4 up to 0.0005 mM stimulates the wild type enzyme 2-3fold. Up to 0.001 mM Gun4 resurrects the inactive gun5 and cch mutant Mg-chelatase reactions to the level seen in the wild type with no Gun4 present, particularly in the case of mutant gun5 | Synechocystis sp. |
Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in Escherichia coli | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
A942V | the (gun5) mutation abolishes activity of Mg-chelatase subunit H. Increasing the ChlH concentration up to 0.02 mM restores approximately 50% activity in the presence of the gun5 mutation. The addition of Gun4 restores Mg-chelatase activity of the gun5 mutant enzyme | Synechocystis sp. |
P595L | the (cch) mutation abolishes activity of Mg-chelatase subunit H. Increasing the ChlH concentration up to 0.02 mM fully restores activity in the presence of the cch mutation. The addition of Gun4 restores Mg-chelatase activity of the cch mutant enzyme | Synechocystis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
1 * 148000 + 1 * 39000 + 1 * 73000, subunits ChlH, ChlI and ChlD | Synechocystis sp. |
73000 | - |
1 * 148000 + 1 * 39000 + 1 * 73000, subunits ChlH, ChlI and ChlD | Synechocystis sp. |
148000 | - |
1 * 148000 + 1 * 39000 + 1 * 73000, subunits ChlH, ChlI and ChlD | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzymes are purified by Ni2+ affinity column chromatography | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protoporphyrin IX + Mg2+ + H2O | - |
Synechocystis sp. | ADP + phosphate + Mg-protoporphyrin IX + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 148000 + 1 * 39000 + 1 * 73000, subunits ChlH, ChlI and ChlD | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
ChlH | subunit of the magnesium chelatase | Synechocystis sp. |
ChlHID | - |
Synechocystis sp. |
Mg-chelatase | - |
Synechocystis sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | magnesium chelatase catalyses the first committed step of chlorophyll biosynthesis | Synechocystis sp. |