Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Chlorobaculum tepidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
protoporphyrin IX | complex of subunits BchD, BchI, BchH2, pH 8.0, 34°C | Chlorobaculum tepidum | |
0.0012 | - |
protoporphyrin IX | complex of subunits BchD, BchI, BchH-1, pH 8.0, 34°C | Chlorobaculum tepidum | |
0.0012 | - |
protoporphyrin IX | complex of subunits BchD, BchI, BchH1, pH 8.0, 34°C | Chlorobaculum tepidum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | K0.5 value 8.6 mM | Chlorobaculum tepidum | |
Mg2+ | K0.5 value 9.1 mM | Chlorobaculum tepidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobaculum tepidum | Q8KB39 | isoform BchH1 | - |
Chlorobaculum tepidum | Q93SV6 | isoform BchH2 | - |
Chlorobaculum tepidum | Q93SV9 | isoform BchH3 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protoporphyrin IX + Mg2+ + H2O | - |
Chlorobaculum tepidum | ADP + phosphate + Mg-protoporphyrin IX + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme interacts with the next enzyme in the pathway, magnesium protoporphyrine IX methyltransferase BchM. Activitiy of BchM in presence of isoform BchH1 is 132% of the activity of BchM alone | Chlorobaculum tepidum |
More | enzyme interacts with the next enzyme in the pathway, magnesium protoporphyrine IX methyltransferase BchM. Activitiy of BchM in presence of isoform BchH2 is 157% of the activity of BchM alone | Chlorobaculum tepidum |
More | enzyme interacts with the next enzyme in the pathway, magnesium protoporphyrine IX methyltransferase BchM. Activitiy of BchM in presence of isoform BchH3 is 68% of the activity of BchM alone | Chlorobaculum tepidum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
protoporphyrin IX | complex of subunits BchD, BchI, BchH1, pH 8.0, 34°C | Chlorobaculum tepidum | |
0.0003 | - |
protoporphyrin IX | complex of subunits BchD, BchI, BchH3, pH 8.0, 34°C | Chlorobaculum tepidum | |
0.03 | - |
protoporphyrin IX | complex of subunits BchD, BchI, BchH2, pH 8.0, 34°C | Chlorobaculum tepidum |