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Literature summary for 6.6.1.1 extracted from

  • Johnson, E.T.; Schmidt-Dannert, C.
    Characterization of three homologs of the large subunit of the magnesium chelatase from Chlorobaculum tepidum and interaction with the magnesium protoporphyrin IX methyltransferase (2008), J. Biol. Chem., 283, 27776-27784.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Chlorobaculum tepidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
protoporphyrin IX complex of subunits BchD, BchI, BchH2, pH 8.0, 34°C Chlorobaculum tepidum
0.0012
-
protoporphyrin IX complex of subunits BchD, BchI, BchH-1, pH 8.0, 34°C Chlorobaculum tepidum
0.0012
-
protoporphyrin IX complex of subunits BchD, BchI, BchH1, pH 8.0, 34°C Chlorobaculum tepidum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ K0.5 value 8.6 mM Chlorobaculum tepidum
Mg2+ K0.5 value 9.1 mM Chlorobaculum tepidum

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum Q8KB39 isoform BchH1
-
Chlorobaculum tepidum Q93SV6 isoform BchH2
-
Chlorobaculum tepidum Q93SV9 isoform BchH3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protoporphyrin IX + Mg2+ + H2O
-
Chlorobaculum tepidum ADP + phosphate + Mg-protoporphyrin IX + H+
-
?

Subunits

Subunits Comment Organism
More enzyme interacts with the next enzyme in the pathway, magnesium protoporphyrine IX methyltransferase BchM. Activitiy of BchM in presence of isoform BchH1 is 132% of the activity of BchM alone Chlorobaculum tepidum
More enzyme interacts with the next enzyme in the pathway, magnesium protoporphyrine IX methyltransferase BchM. Activitiy of BchM in presence of isoform BchH2 is 157% of the activity of BchM alone Chlorobaculum tepidum
More enzyme interacts with the next enzyme in the pathway, magnesium protoporphyrine IX methyltransferase BchM. Activitiy of BchM in presence of isoform BchH3 is 68% of the activity of BchM alone Chlorobaculum tepidum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
protoporphyrin IX complex of subunits BchD, BchI, BchH1, pH 8.0, 34°C Chlorobaculum tepidum
0.0003
-
protoporphyrin IX complex of subunits BchD, BchI, BchH3, pH 8.0, 34°C Chlorobaculum tepidum
0.03
-
protoporphyrin IX complex of subunits BchD, BchI, BchH2, pH 8.0, 34°C Chlorobaculum tepidum