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Literature summary for 6.6.1.1 extracted from

  • Petersen, B.L.; Jensen, P.E.; Gibson, L.C.D.; Stummann, B.M.; Hunter, C.N.; Henningsen, K.W.
    Reconstitution of an active magnesium chelatase enzyme complex from the bchI, -D, and -H gene products of the green sulfur bacterium Chlorobium vibrioforme expressed in Escherichia coli (1998), J. Bacteriol., 180, 699-704.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Prosthecochloris vibrioformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 38000, SDS-PAGE, a construct expressing I subunit Prosthecochloris vibrioformis
42000
-
x * 42000, SDS-PAGE, a construct expressing I subunit Prosthecochloris vibrioformis

Organism

Organism UniProt Comment Textmining
Prosthecochloris vibrioformis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ subunits H, I and D from bchH, bchI and bchD genes combine to form the enzyme complex, the complex is active only when the three proteins are present Prosthecochloris vibrioformis
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Prosthecochloris vibrioformis

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE, a construct expressing I subunit Prosthecochloris vibrioformis
? x * 42000, SDS-PAGE, a construct expressing I subunit Prosthecochloris vibrioformis
More the enzyme has three subunits, BchI, BchH and BchD Prosthecochloris vibrioformis