Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.6.1.1 extracted from

  • Gorchein, A.
    Cell-free activity of magnesium chelatase in Rhodobacter spheroides and Rhodobacter capsulatus (1997), Biochem. Soc. Trans., 25, 82S.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
thiomerosal 60% inhibition with 0.022 mM, in the absence of DTT Cereibacter sphaeroides
thiomerosal 60% inhibition with 0.022 mM, in the absence of DTT Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00089
-
protoporphyrin IX pH 8.2, 20-22ºC, 192000 * g supernatant Cereibacter sphaeroides
0.00089
-
protoporphyrin IX pH 8.2, 20-22ºC, 192000 * g supernatant Rhodobacter capsulatus
0.00283
-
protoporphyrin IX pH 8.2, 20-22ºC, 20000 * g supernatant Cereibacter sphaeroides
0.00283
-
protoporphyrin IX pH 8.2, 20-22ºC, 20000 * g supernatant Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + protoporphyrin IX + Mg2+ + H2O Rhodobacter capsulatus
-
ADP + phosphate + Mg-protoporphyrin IX + H+
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Rhodobacter capsulatus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Cereibacter sphaeroides
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protoporphyrin IX + Mg2+ + H2O
-
Rhodobacter capsulatus ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O optimum activity at 11.5 mM Mg2+ Cereibacter sphaeroides ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O optimum activity at 11.5 mM Mg2+ Rhodobacter capsulatus ADP + phosphate + Mg-protoporphyrin IX + H+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 22 assay at Cereibacter sphaeroides
20 22 assay at Rhodobacter capsulatus