Inhibitors | Comment | Organism | Structure |
---|---|---|---|
thiomerosal | 60% inhibition with 0.022 mM, in the absence of DTT | Cereibacter sphaeroides | |
thiomerosal | 60% inhibition with 0.022 mM, in the absence of DTT | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00089 | - |
protoporphyrin IX | pH 8.2, 20-22ºC, 192000 * g supernatant | Cereibacter sphaeroides | |
0.00089 | - |
protoporphyrin IX | pH 8.2, 20-22ºC, 192000 * g supernatant | Rhodobacter capsulatus | |
0.00283 | - |
protoporphyrin IX | pH 8.2, 20-22ºC, 20000 * g supernatant | Cereibacter sphaeroides | |
0.00283 | - |
protoporphyrin IX | pH 8.2, 20-22ºC, 20000 * g supernatant | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protoporphyrin IX + Mg2+ + H2O | Rhodobacter capsulatus | - |
ADP + phosphate + Mg-protoporphyrin IX + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Rhodobacter capsulatus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ | this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway | Cereibacter sphaeroides | |
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ | this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protoporphyrin IX + Mg2+ + H2O | - |
Rhodobacter capsulatus | ADP + phosphate + Mg-protoporphyrin IX + H+ | - |
? | |
ATP + protoporphyrin IX + Mg2+ + H2O | optimum activity at 11.5 mM Mg2+ | Cereibacter sphaeroides | ADP + phosphate + Mg-protoporphyrin IX + H+ | - |
? | |
ATP + protoporphyrin IX + Mg2+ + H2O | optimum activity at 11.5 mM Mg2+ | Rhodobacter capsulatus | ADP + phosphate + Mg-protoporphyrin IX + H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 22 | assay at | Cereibacter sphaeroides |
20 | 22 | assay at | Rhodobacter capsulatus |