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Literature summary for 6.6.1.1 extracted from

  • Walker, C.J.; Willows, R.D.
    Mechanism and regulation of Mg-chelatase (1997), Biochem. J., 327, 321-333.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
Cereibacter sphaeroides
ATP
-
Rhodobacter capsulatus
ATP required for activation and chelation steps Pisum sativum

Cloned(Commentary)

Cloned (Comment) Organism
expression of bchD gene in Escherichia coli Cereibacter sphaeroides
expression of bchD gene in Escherichia coli Rhodobacter capsulatus

General Stability

General Stability Organism
high concentrations of protoporphyrin, ATP and Mg2+ during gentle lysis, stabilise Cucumis sativus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Cucumis sativus 16020
-
additional information multicomponent enzyme, requires at least two proteins, one membrane-associated and one soluble Pisum sativum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
x * 46000, calculation from sequence of cDNA, I subunit Arabidopsis thaliana
60000
-
x * 60000, calculation from sequence of cDNA, D subunit Synechocystis sp.
73000
-
x * 73000, calculation from sequence of cDNA, D subunit Rhodobacter capsulatus
87000
-
x * 87000, calculation from sequence of cDNA, D subunit Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + protoporphyrin IX + Mg2+ + H2O Hordeum vulgare
-
ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O Pisum sativum
-
ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O Arabidopsis thaliana
-
ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O Rhodobacter capsulatus
-
ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O Cucumis sativus
-
ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O Synechocystis sp.
-
ADP + phosphate + Mg-protoporphyrin IX + H+
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Cereibacter sphaeroides
-
-
-
Cucumis sativus
-
-
-
Hordeum vulgare
-
-
-
Pisum sativum
-
-
-
Rhodobacter capsulatus
-
-
-
Synechocystis sp. P51634
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ subunits H, I and D from bchH, bchI and bchD genes combine to form the enzyme complex, the complex is active only when the three proteins are present Cereibacter sphaeroides
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ subunits H, I and D from bchH, bchI and bchD genes combine to form the enzyme complex, the complex is active only when the three proteins are present Arabidopsis thaliana
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ subunits H, I and D from bchH, bchI and bchD genes combine to form the enzyme complex, the complex is active only when the three proteins are present Rhodobacter capsulatus
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Cereibacter sphaeroides
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Pisum sativum
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Arabidopsis thaliana
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Rhodobacter capsulatus
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Cucumis sativus
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Synechocystis sp.
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Cereibacter sphaeroides
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Hordeum vulgare
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Pisum sativum
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Arabidopsis thaliana
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Rhodobacter capsulatus
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Cucumis sativus
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protoporphyrin IX + Mg2+ + H2O
-
Cereibacter sphaeroides ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O
-
Hordeum vulgare ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O
-
Pisum sativum ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O
-
Arabidopsis thaliana ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O
-
Rhodobacter capsulatus ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O
-
Cucumis sativus ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O
-
Synechocystis sp. ADP + phosphate + Mg-protoporphyrin IX + H+
-
?

Subunits

Subunits Comment Organism
?
-
Hordeum vulgare
? x * 73000, calculation from sequence of cDNA, D subunit Rhodobacter capsulatus
? x * 46000, calculation from sequence of cDNA, I subunit Arabidopsis thaliana
? x * 87000, calculation from sequence of cDNA, D subunit Cereibacter sphaeroides
? x * 60000, calculation from sequence of cDNA, D subunit Synechocystis sp.
More
-
Synechocystis sp.
More the enzyme has three subunits, BchI, BchH and BchD Cereibacter sphaeroides
More the enzyme has three subunits, BchI, BchH and BchD Rhodobacter capsulatus
More H subunit is a monomer and I subunit is a dimer, determined by dynamic-light-scattering studies Pisum sativum
More the enzyme has three subunits, ChlI, ChlH and ChlD Hordeum vulgare
More the enzyme has three subunits, ChlI, ChlH and ChlD Pisum sativum