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Literature summary for 6.6.1.1 extracted from

  • Walker, C.J.; Weinstein, J.D.
    In vitro assay of the chlorophyll biosynthetic enzyme Mg-chelatase: resolution of the activity into soluble and membrane-bound fractions (1991), Proc. Natl. Acad. Sci. USA, 88, 5789-5793.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
26% loss of activity in chloroplasts subjected to hypotonic lysis and freeze-thaw cycles Pisum sativum

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide potent, IC50 of 0.02 mM Pisum sativum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Pisum sativum 9507
-
chloroplast
-
Cucumis sativus 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pisum sativum
Mg2+ required Cucumis sativus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + protoporphyrin IX + Mg2+ + H2O Pisum sativum
-
ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O Cucumis sativus
-
ADP + phosphate + Mg-protoporphyrin IX + H+
-
?

Organism

Organism UniProt Comment Textmining
Cucumis sativus
-
Sumter var
-
Pisum sativum
-
pea
-
Pisum sativum
-
var Bolero
-
Pisum sativum
-
var Spring
-

Reaction

Reaction Comment Organism Reaction ID
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Pisum sativum
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Cucumis sativus
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Pisum sativum
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Cucumis sativus
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ linear reaction for at least 60 min under standard incubation Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Cucumis sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
3fold to 4fold higher values than in cucumber chloroplasts Pisum sativum
0.0000024
-
pellet of broken fractionated chloroplasts Pisum sativum
0.0000026
-
supernatant of broken fractionated chloroplasts Pisum sativum
0.00002
-
broken unfractionated chloroplasts Pisum sativum
0.0001
-
intact chloroplasts Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protoporphyrin IX + Mg2+ + H2O
-
Pisum sativum ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O
-
Cucumis sativus ADP + phosphate + Mg-protoporphyrin IX + H+
-
?
ATP + protoporphyrin IX + Mg2+ + H2O omission of any of the substrates results in complete loss of activity. Optimum concentration of Mg2+ is lower for intact than broken and reconstituted chloroplasts Pisum sativum ADP + phosphate + Mg-protoporphyrin IX + H+
-
?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.02
-
potent, IC50 of 0.02 mM Pisum sativum N-ethylmaleimide