Literature summary for 6.5.1.4 extracted from

Chakravarty, A.K.; Shuman, S.
RNA 3'-phosphate cyclase (RtcA) catalyzes ligase-like adenylylation of DNA and RNA 5'-monophosphate ends (2011), J. Biol. Chem., 286, 4117-4122.

Search for more literature for 6.5.1.4

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity, 2 mM Mg2+ is used in assay conditions	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + DNA 5'-terminal-phosphate	RtcA also catalyzes adenylylation of 5'-phosphate ends of DNA strands to form AppDNA product	Escherichia coli	AMP + AppDNA	-	?
ATP + RNA 3'-terminal-phosphate	RtcA catalyzes the synthesis of RNA 2',3'-cyclic phosphate ends via an ATP-dependent pathway comprising three nucleotidyl transfer steps: reaction of RtcA with ATP to form a covalent RtcA-(histidinyl-N)-AMP intermediate and release diphosphate, transfer of AMP from RtcA to an RNA 3'-phosphate to form an RNA(3')pp(5')A intermediate, and attack by the terminal nucleoside O2' on the 3'-phosphate to form an RNA 2',3'-cyclic phosphate product and release AMP	Escherichia coli	AMP + diphosphate + RNA terminal-2',3'-cyclic phosphate	-	?
ATP + RNA 5'-terminal-phosphate	RtcA also catalyzes adenylylation of 5'-phosphate ends of RNA strands to form AppRNA product	Escherichia coli	AMP + AppRNA	-	?
additional information	RtcA readily adenylylates the 5'-phosphate at a 5'-PO4/3'-OH nick in duplex DNA but is unable to covert the nicked DNA-adenylate to a sealed phosphodiester	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
RNA 3'-phosphate cyclase	-	Escherichia coli
RtcA	-	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	6	pH-optimum for DNA 5'-adenylylation Tris acetate buffer	Escherichia coli
8	8.5	pH-optimum for RNA 3'-cyclization	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

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Release 2023.1 (January 2023)