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Literature summary for 6.5.1.3 extracted from

  • Chauleau, M.; Shuman, S.
    Kinetic mechanism of nick sealing by T4 RNA ligase 2 and effects of 3-OH base mispairs and damaged base lesions (2013), RNA, 19, 1840-1847.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
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-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m the kinetic mechanism of single-turnover nick sealing by T4 Rnl2-AMP is explored by using a rapid mix-quench method and the effects of 3'-OH mispairs and base damage lesions on the rates of nick 5'-adenylylation and phosphodiester synthesis is determined. With respect to the sealing of perfectly paired nicks the rates of step 2 catalysis are rapid (9.5-17.9/sec) and similar in magnitude to the step 3 rates (7.9-32/sec). Rnl2 is kinetically sensitive to all 3'-OH base mispairs Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
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Synonyms

Synonyms Comment Organism
Rnl2
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 Tequatrovirus T4
T4 RNA ligase 2
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 Tequatrovirus T4

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information the kinetic mechanism of single-turnover nick sealing by T4 Rnl2-AMP is explored by using a rapid mix-quench method and the effects of 3'-OH mispairs and base damage lesions on the rates of nick 5'-adenylylation and phosphodiester synthesis is determined. With respect to the sealing of perfectly paired nicks the rates of step 2 catalysis are rapid (9.5-17.9/sec) and similar in magnitude to the step 3 rates (7.9-32/sec). Rnl2 is kinetically sensitive to all 3'-OH base mispairs Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
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 assay at Tequatrovirus T4