Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanothermobacter thermautotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of N-terminal amino acids 1-253 gives a protein defective in overall ligation but retaining the ability to form EpA intermediate, bind to pRNA and transfer AMP to pRNA, albeit less efficiently than wild-type ligase. Expression of amino acids 255-381 results in a protein that fails to form a detectable protein-RNA complex and does not support overall ligation or RNA circularization | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, calculated and sedimentation analysis | Methanothermobacter thermautotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(deoxyribonucleotide)n | - |
Methanothermobacter thermautotrophicus | circular (deoxyribonucleotide)n | - |
? | |
(ribonucleotide)n | - |
Methanothermobacter thermautotrophicus | circular (ribonucleotide)n | - |
? | |
ATP + (deoxyribonucleotide)n | - |
Methanothermobacter thermautotrophicus | adenylyl-(deoxyribonucleotide)n + diphosphate | - |
? | |
ATP + (ribonucleotide)n | - |
Methanothermobacter thermautotrophicus | adenylyl-(ribonucleotide)n + diphosphate | - |
? | |
additional information | enzyme does not discriminate between RNA and DNA for phosphodiester bond formation | Methanothermobacter thermautotrophicus | ? | - |
? | |
additional information | intramolecular ligation of 5'-PO4 single-strand RNA to form a covalently closed circular RNA molecule through ligase-adenylylate and RNA-adenylylate intermediates AppRNA. At the optimal temperature of 65°C, AppRNA is predominantly ligated to a circular product. At 35°C, phosphodiester bond formation is suppressed and the majority of the AppRNA is deadenylylated | Methanothermobacter thermautotrophicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, calculated and sedimentation analysis | Methanothermobacter thermautotrophicus |
More | the C-terminal 127 amino acid segment is required for dimerization | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
RNA-adenylylate intermediates AppRNA are predominantly ligated to a circular product | Methanothermobacter thermautotrophicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
phosphodiester bond formation is suppressed and the majority of the RNA-adenylylate intermediates AppRNA are deadenylylated | Methanothermobacter thermautotrophicus |