Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.5.1.3 extracted from

  • Wang, L.K.; Nandakumar, J.; Schwer, B.; Shuman, S.
    The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair (2007), RNA, 13, 1235-1244.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.3

Protein Variants

Protein Variants Comment Organism
H250A pRNA circularization by the mutant enzyme is 6% of the wild-type rate Tequatrovirus T4
K117A mutation is lethal in vivo Tequatrovirus T4
L104A L104A strain is temperature sensitive, no growth at 37°C Tequatrovirus T4
L104A pRNA circularization by the mutant enzyme is 6% of the wild-type rate Tequatrovirus T4
additional information S121A, S124A, and H250 strains grow at all temperatures Tequatrovirus T4
S103A pRNA circularization by the mutant enzyme is 5% of the wild-type rate Tequatrovirus T4
S103A S103A strain is temperature sensitive, no growth at 37°C Tequatrovirus T4
S118A S118 strain is temperature sensitive, no growth at 37°C Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m Rnl1 has an inherent specificity for sealing tRNA with a break in the anticodon loop. The tRNA specificity is imparted by the C domain Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Synonyms

Synonyms Comment Organism
RNA ligase 1
-
 Tequatrovirus T4
Rnl1
-
 Tequatrovirus T4