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Literature summary for 6.5.1.3 extracted from

  • Ekland, E.H.; Szostak, J.W.; Bartel, D.P.
    Structurally complex and highly active RNA ligases derived from random RNA sequences (1995), Science, 269, 364-370.
    View publication on PubMed
Search for more literature for 6.5.1.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 (ribonucleotide)n+m pH 8.0, 22°C synthetic construct

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m synthetic construct
-
 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Organism

Organism UniProt Comment Textmining
synthetic construct
-
 isolated from random RNA sequences
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m
-
 synthetic construct AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Synonyms

Synonyms Comment Organism
b1-10t
-
 synthetic construct
class I ligase
-
 synthetic construct

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.66
-
 (ribonucleotide)n+m pH 8.0, 22°C synthetic construct
2.94
-
 (ribonucleotide)n+m pH 8.0, 22°C synthetic construct