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Literature summary for 6.5.1.3 extracted from

  • Glasner, M.E.; Bergman, N.H.; Bartel, D.P.
    Metal ion requirements for structure and catalysis of an RNA ligase ribozyme (2002), Biochemistry, 41, 8103-8112.
    View publication on PubMed
Search for more literature for 6.5.1.3

Cloned(Commentary)

Cloned (Comment) Organism
-
 synthetic construct

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 synthetic construct
Ca2+
-
 synthetic construct
Cd2+
-
 synthetic construct
Co(NH3)63+
-
 synthetic construct
Co2+
-
 synthetic construct
Mn2+
-
 synthetic construct
Pb2+
-
 synthetic construct
spermine
-
 synthetic construct
Sr2+
-
 synthetic construct
Zn2+
-
 synthetic construct

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requires metal ions for structure and catalysis synthetic construct
Mn2+ only marginal activity synthetic construct
additional information no detectable activity with Ca2+, Sr2+, Ba2+, Zn2+, Co2+, Cd2+, Pb2+, Co(NH3)6 3+, or spermine synthetic construct

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m synthetic construct
-
 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Organism

Organism UniProt Comment Textmining
synthetic construct
-
 isolated from random sequence
-

Purification (Commentary)

Purification (Comment) Organism
-
 synthetic construct

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m
-
 synthetic construct AMP + diphosphate + (ribonucleotide)n+m
-
 ?
ATP + 5'-aaaCCAGUC RNA-DNA hybrid as substrate, chemical step is represented as irreversible, because reverse reaction, pyrophosphorolysis with formation of a triphosphate is 10000000-times slower than the forward reaction synthetic construct AMP + diphosphate + ?
-
 ir

Synonyms

Synonyms Comment Organism
class I ligase
-
 synthetic construct
RNA ligase ribozyme
-
 synthetic construct

pH Range

pH Minimum pH Maximum Comment Organism
5.7 7
-
 synthetic construct