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Literature summary for 6.5.1.3 extracted from

  • Maunders, M.J.
    DNA and RNA ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3) (1993), Methods Mol. Biol., 16, 213-230.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000 48200 gel filtration, high-speed sedimentation equilibrium centrifugation Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Storage Stability

Storage Stability Organism
-20°C Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m
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Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
?
dATP + (ribonucleotide)n + (ribonucleotide)m acts on single-stranded or double-stranded DNA molecules. Acts on very small pieces of ribonucleic acid, with 40mers being the probable upper size limit. The minimum size of the 5'-moiety is a ribonucleoside 3',5'-bisphosphate Tequatrovirus T4 dAMP + phosphate + (ribonucleotide)n+m
-
?