Cloned (Comment) | Organism |
---|---|
overexpressed as a His-tagged fusion protein in Escherichia coli | Wolbachia endosymbiont of Brugia malayi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000193 | - |
deoxyribonucleotide | pH 8, 25°C | Wolbachia endosymbiont of Brugia malayi | |
0.0048 | - |
NAD+ | pH 8, 25°C | Wolbachia endosymbiont of Brugia malayi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | no activity observed in the presence of | Wolbachia endosymbiont of Brugia malayi | |
KCl | enzymatic activity increased in the presence of monovalent cations, maximal activity at 25 mM NaCl | Wolbachia endosymbiont of Brugia malayi | |
MgCl2 | highest activity: 10 mM MgCl2 | Wolbachia endosymbiont of Brugia malayi | |
NaCl | enzymatic activity increased in the presence of monovalent cations, maximal activity at 5 mM NaCl | Wolbachia endosymbiont of Brugia malayi | |
NH4Cl | enzymatic activity increased in the presence of monovalent cations, maximal activity at 10 mM NaCl | Wolbachia endosymbiont of Brugia malayi | |
Ni2+ | no activity observed in the presence of | Wolbachia endosymbiont of Brugia malayi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
SDS-PAGE, recombinant protein | Wolbachia endosymbiont of Brugia malayi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Wolbachia endosymbiont of Brugia malayi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Wolbachia endosymbiont of Brugia malayi |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + deoxyribonucleotide | - |
Wolbachia endosymbiont of Brugia malayi | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
wBm-LigA | - |
Wolbachia endosymbiont of Brugia malayi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Wolbachia endosymbiont of Brugia malayi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Wolbachia endosymbiont of Brugia malayi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Wolbachia endosymbiont of Brugia malayi |
Organism | Comment | Expression |
---|---|---|
Wolbachia endosymbiont of Brugia malayi | high enzyme expression is observed in Wolbachia within Brugia malayi microfilariae and female adult parasites along the hypodermal chords and in the gravid portion | up |