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Literature summary for 6.5.1.2 extracted from

  • Jahic, H.; Liu, C.F.; Thresher, J.; Livchak, S.; Wang, H.; Ehmann, D.E.
    The kinetic mechanism of S. pneumoniae DNA ligase and inhibition by adenosine-based antibacterial compounds (2012), Biochem. Pharmacol., 84, 654-660.
    View publication on PubMed
Search for more literature for 6.5.1.2

Protein Variants

Protein Variants Comment Organism
L75F L75F mutant is as catalytically efficient as wild-type, athough the NAD Km is slightly elevated compared to wild-type, it is still likely well below the concentration of NAD in the bacterium. IC50 for inhibitors elevated compared to wild-type Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
(2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
-
 Streptococcus pneumoniae
(2R,3R,4S,5R)-2-[6-amino-2-(cyclopentyloxy)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
-
 Streptococcus pneumoniae
5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine
-
 Streptococcus pneumoniae
deoxyribonucleotide
-
 Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000028
-
 deoxyribonucleotide co-substrate: NAD+, mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae
0.000028
-
 deoxyribonucleotide wild-type, 25°C, pH 7.5 Streptococcus pneumoniae
0.004
-
 NAD+ wild-type, 25°C, pH 7.5 Streptococcus pneumoniae
0.026
-
 NAD+ mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + deoxyribonucleotide Streptococcus pneumoniae
-
 AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + deoxyribonucleotide
-
 Streptococcus pneumoniae AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

Synonyms Comment Organism
NAD-dependent DNA ligase
-
 Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.27
-
 deoxyribonucleotide co-substrate: NAD+, mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae
0.27
-
 deoxyribonucleotide co-substrate: NAD+, wild-type, 25°C, pH 7.5 Streptococcus pneumoniae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000051
-
 deoxyribonucleotide wild-type, 25°C, pH 7.5 Streptococcus pneumoniae
0.000065
-
 deoxyribonucleotide co-substrate: NAD+, mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00004
-
 wild-type, 25°C, pH 7.5 Streptococcus pneumoniae 5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine
0.0001
-
 wild-type, 25°C, pH 7.5 Streptococcus pneumoniae (2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
0.0002
-
 wild-type, 25°C, pH 7.5 Streptococcus pneumoniae (2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
0.01
-
 mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae (2R,3R,4S,5R)-2-[6-amino-2-(butylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol
0.012
-
 mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae 5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine
0.03
-
 mutant L75F, 25°C, pH 7.5 Streptococcus pneumoniae (2R,3R,4S,5R)-2-[6-amino-2-(cyclopentyloxy)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol