Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme requires a divalent cation like Ca2+, optimal activity at about 20 mM Ca2+ | Thermotoga maritima | |
Mg2+ | the enzyme requires a divalent cation like Mg2+, optimal activity at about 20 mM Mg2+ | Thermotoga maritima | |
Mn2+ | the enzyme requires a divalent cation like Mn2+, maximal activity at 3 mM Mn2+ | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, SDS-PAGE | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Thermotoga maritima ATCC 43589 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heat treatment and immobilized metal affinity column chromatography | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | purified DNA ligase exhibits activity on DNA fragments with cohesive termini, and no activity is detected on blunt-end DNA. DNA ligase promotes the amplification of long DNA fragments from the genomic DNA of Thermotoga maritima | Thermotoga maritima | ? | - |
? | |
additional information | purified DNA ligase exhibits activity on DNA fragments with cohesive termini, and no activity is detected on blunt-end DNA. DNA ligase promotes the amplification of long DNA fragments from the genomic DNA of Thermotoga maritima | Thermotoga maritima ATCC 43589 | ? | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Hind III digested plasmid | Thermotoga maritima | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Hind III digested plasmid | Thermotoga maritima ATCC 43589 | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
LigA | - |
Thermotoga maritima |
NAD+-dependent DNA ligase | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
with Hind III digested plasmid as substrate | Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
the purified enzyme has a half-life of over 30 min at 95°C | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
with Hind III digested plasmid as substrate | Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Thermotoga maritima |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.8 | the enzyme retains over 80% of its activity after holding a pH ranging from 7.2 to 8.8 for 1 h at 80°C | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | does not require ATP as cofactor | Thermotoga maritima | |
NAD+ | required, optimal activity at 1 mM NAD+ | Thermotoga maritima |