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Literature summary for 6.5.1.2 extracted from

  • Le, D.; Hua, X.; Huang, L.; Gao, G.; Lu, H.; Xu, Z.; Tian, B.; Hua, Y.
    Biochemical characterization of two DNA ligases from Deinococcus radiodurans (2008), Protein Pept. Lett., 15, 600-605.
    View publication on PubMed
Search for more literature for 6.5.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Deinococcus radiodurans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum concentration at 5 mM Deinococcus radiodurans

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Deinococcus radiodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Deinococcus radiodurans AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

Synonyms Comment Organism
LigA
-
 Deinococcus radiodurans
NAD(+)-dependent DNA ligase
-
 Deinococcus radiodurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Deinococcus radiodurans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 the half-life of heat inactivation at 100°C is about 3 min Deinococcus radiodurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Deinococcus radiodurans

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Deinococcus radiodurans