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Literature summary for 6.5.1.2 extracted from

  • Panasenko, S.M.; Alazard, R.J.; Lehman, I.R.
    A simple, three-step procedure for the large scale purification of DNA ligase from a hybrid lysogen constructed in vitro (1978), J. Biol. Chem., 253, 4590-4592.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Escherichia coli
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strain 595 (lamdagt4 lop11 lig+ S7) with 500fold higher activity than the wild type strain
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Purification (Commentary)

Purification (Comment) Organism
large scale Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
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Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
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