Literature summary for 6.5.1.1 extracted from

Kim, J.H.; Lee, K.K.; Sun, Y.; Seo, G.J.; Cho, S.S.; Kwon, S.H.; Kwon, S.T.
Broad nucleotide cofactor specificity of DNA ligase from the hyperthermophilic crenarchaeon Hyperthermus butylicus and its evolutionary significance (2013), Extremophiles, 17, 515-522.

Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
Hbu DNA ligase gene is expressed under control of the T7lac promoter of pTARG in Escherichia coli BL21-Codon-Plus(DE3)-RIL	Hyperthermus butylicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Hyperthermus butylicus
Mn2+	required	Hyperthermus butylicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
68000	-	SDS-PAGE	Hyperthermus butylicus

Organism

Organism	UniProt	Comment	Textmining
Hyperthermus butylicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified using the IMPACTTM-CN system (intein-mediated purification with an affinity chitin-binding tag) and cation-ion (Arg-tag) chromatography	Hyperthermus butylicus

Synonyms

Synonyms	Comment	Organism
Hbu DNA ligase	-	Hyperthermus butylicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	-	Hyperthermus butylicus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
94	-	Hbu DNA ligase exhibits about 50% of the original activity for 2.3 h at temperatures up to 94 °C	Hyperthermus butylicus
99	-	Hbu DNA ligase exhibtis a 1.7 h half life at 99 °C	Hyperthermus butylicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Hyperthermus butylicus

Cofactor

Cofactor	Comment	Organism	Structure
ADP	-	Hyperthermus butylicus
ATP	-	Hyperthermus butylicus
GTP	-	Hyperthermus butylicus
additional information	enzyme shows a broad nucleotide cofactor specificity	Hyperthermus butylicus

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Release 2023.1 (January 2023)