Literature summary for 6.5.1.1 extracted from

Tanabe, M.; Ishino, S.; Ishino, Y.; Nishida, H.
Mutations of Asp540 and the domain-connecting residues synergistically enhance Pyrococcus furiosus DNA ligase activity (2014), FEBS Lett., 588, 230-235.

Search for more literature for 6.5.1.1

Protein Variants

Protein Variants	Comment	Organism
D540R/delC4	DelC4 i.e. deletion of the four C-terminal residues, nick-joining activities of the mutant is enhanced as compared to that of the D540R single substitution	Pyrococcus furiosus
D540R/DELTAC4	the combination of the Asp540-replacement and the elimination of ionic residues in the helix, forming interactions with adenylylation domain, effectively enhances the activity	Pyrococcus furiosus
D540R/K554A/K558A	the combination of the Asp540-replacement and the elimination of ionic residues in the helix, forming interactions with adenylylation domain, effectively enhances the activity	Pyrococcus furiosus
D540R/K554A/K558A	nick-joining activities of the mutant is enhanced, as compared to that of the D540R single substitution	Pyrococcus furiosus
additional information	combination of the D540R-replacement and the elimination of ionic residues in the helix, forming interactions with AdD, effectively enhances the activity	Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	Pyrococcus furiosus	-	AMP + diphosphate + (deoxyribonucleotide)n+m	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	P56709	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Pyrococcus furiosus	AMP + diphosphate + (deoxyribonucleotide)n+m	-	?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	annealing of two short oligonucleotides, a 5'-phosphate-terminated strand 30mer (phosphate-5'-AGGTCGACTCCAGAGGATTGTTGACCGGCC-3') and a 5'-TET labeled 20mer (TET-5'-CGCCAAGCTTGCATTCCTAC-3'), to a 40mer complementary oligonucleotide target (5'-CAATCCTCTGGAGTCGACCTGTAGGAATGCAAGCTTGGCG-3')	Pyrococcus furiosus	AMP + diphosphate + (deoxyribonucleotide)n+m	-	?

Synonyms

Synonyms	Comment	Organism
PF1635	locus name	Pyrococcus furiosus
PfuLig	-	Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	assay at	Pyrococcus furiosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Pyrococcus furiosus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)